﻿258 
  BOTANICAL 
  GAZETTE. 
  [ 
  October, 
  

  

  Muscat 
  vine 
  were 
  inoculated 
  with 
  pycnidia-spores 
  obtained 
  

   from 
  another 
  house 
  grown 
  Muscat 
  and 
  immediately 
  covered 
  

   with 
  a 
  bell 
  jar. 
  The 
  spores 
  were 
  obtained 
  in 
  the 
  usual 
  way 
  

   by 
  teasing 
  up 
  a 
  bit 
  of 
  the 
  affected 
  leaf 
  in 
  distilled 
  water. 
  In 
  

   ten 
  days 
  the 
  inoculated 
  leaves 
  were 
  all 
  affected. 
  The 
  leaves 
  

   of 
  one 
  of 
  the 
  control 
  plants 
  also 
  showed 
  the 
  characteristic 
  

   spots 
  of 
  the 
  Phyllosticta, 
  which 
  fact 
  throws 
  a 
  doubt 
  on 
  the 
  

   whole 
  experiment. 
  Unfortunately, 
  we 
  were 
  not 
  able 
  to 
  re- 
  

   peat 
  this 
  trial, 
  as 
  no 
  plants 
  suitable 
  for 
  the 
  purpose 
  could 
  be 
  

   obtained. 
  

  

  Summing 
  up 
  briefly 
  the 
  positive 
  results 
  of 
  these 
  investi- 
  

   gations, 
  we 
  have 
  the 
  following 
  : 
  

  

  I. 
  Two 
  hundred 
  or 
  more 
  inoculations 
  of 
  the 
  grape 
  berry 
  

   with 
  pycnidia-spores 
  from 
  the 
  leaves 
  of 
  Vitis 
  and 
  Ampelopsis 
  

   produced 
  no 
  effect 
  whatever. 
  , 
  

  

  II. 
  The 
  same 
  number 
  of 
  inoculations 
  of 
  the 
  leaves 
  ot 
  

   Vitis 
  and 
  Ampelopsis 
  with 
  pycnidia-spores 
  from 
  the 
  bernes 
  

   of 
  the 
  grape 
  gave 
  the 
  same 
  results 
  as 
  I. 
  

  

  III. 
  Fifty 
  or 
  more 
  inoculations 
  of 
  the 
  berries 
  with 
  pyc- 
  

   nidia-spores 
  from 
  the 
  berries 
  yielded 
  the 
  same 
  results 
  as 
  

   and 
  II. 
  

  

  IV. 
  Inoculation 
  of 
  Ampelopsis 
  leaves 
  with 
  ascospores 
  

   frorn 
  grape 
  berries 
  resulted 
  in 
  the 
  formation 
  of 
  typical 
  rh) 
  

   losticta 
  ampelopsidis 
  spots, 
  pycnidia 
  and 
  spores 
  at 
  the 
  expii" 
  ■ 
  

   tion 
  of 
  fifteen 
  days. 
  

  

  V. 
  Inoculations 
  of 
  grape 
  leaves 
  with 
  ascospores 
  nj 
  

   the 
  berry 
  produced 
  Phyllosticta 
  labruscai 
  spots, 
  pycnia 
  

   and 
  spores 
  in 
  twenty-five 
  days. 
  jp 
  

  

  We 
  have 
  no 
  reason 
  to 
  doubt 
  the 
  genuineness 
  of 
  thejes 
  

   obtained 
  in 
  the 
  case 
  of 
  IV 
  and 
  V, 
  first, 
  because 
  the 
  old 
  bern 
  - 
  

   so 
  far 
  as 
  could 
  be 
  determined 
  by 
  microscopic 
  examinan 
  • 
  

   contained 
  no 
  other 
  reproductive 
  bodies 
  but 
  the 
  ^scospo 
  ; 
  

   and, 
  second, 
  an 
  examination 
  of 
  the 
  water 
  found 
  ontheie_ 
  

   after 
  a 
  rain 
  revealed 
  only 
  ascospores, 
  and 
  these 
  m 
  ^a 
  . 
  

   stages 
  of 
  germination. 
  So 
  far 
  as 
  we 
  know 
  no 
  other 
  atre 
  t^ 
  

   of 
  this 
  kind 
  has 
  been 
  made 
  to 
  establish 
  the 
  identity 
  .^ 
  

   various 
  forms 
  here 
  discussed, 
  excepting 
  that 
  by 
  via 
  

   France 
  in 
  1888.^ 
  In 
  May 
  of 
  that 
  year, 
  according 
  to 
  V 
  J 
  

   published 
  statement, 
  the 
  ascospores 
  of 
  the 
  black- 
  rot 
  tui^_^ 
  

  

  !5!.u^. 
  !°^y^ 
  °^ 
  healthy 
  grape 
  leaves 
  still 
  on 
  the 
  vine, 
  ^^^^j^^ 
  ^, 
  

   the 
  Phyllosticta 
  apVeared7'"ProfrViara 
  Toes 
  not 
  sayho^' 
  

  

  . 
  1 
  -"-*^c*xLnj 
  giapc 
  leaves 
  stiii 
  «^ia 
  ^^^^ 
  ' 
  Hile^ 
  ^ 
  

  

  fu^ 
  ii.^^,^''^!'^^ 
  ^'^ys 
  the 
  characteristic 
  spots 
  and 
  P"^"^ 
  the 
  

  

  inoculated 
  leaves 
  wereprotected 
  ifrorn'outside 
  contaminatt^"^ 
  

  

  dela 
  vig?e!'' 
  ^"""^''"^ 
  '^^' 
  '''^' 
  ^^^* 
  ^- 
  ^'^'^ 
  I^eeherches 
  experimeatalessurles 
  

  

  