﻿

  1890.] 
  BOTANICAI. 
  GAZETTK. 
  293 
  

  

  more. 
  The 
  prepared 
  plates, 
  or 
  fragments, 
  should 
  be 
  trans- 
  

   parent, 
  of 
  a 
  light 
  yellow 
  color, 
  very 
  tough 
  and 
  elastic 
  ; 
  if 
  

   brittle 
  and 
  possessing 
  a 
  milky 
  opaqueness, 
  it 
  is 
  of 
  an 
  inferior 
  

   grade 
  and 
  the 
  imbedding 
  mass 
  will 
  be 
  too 
  brittle 
  or 
  soft. 
  

  

  The 
  fragments 
  are 
  enclosed 
  in 
  an 
  airtight 
  chamber; 
  a 
  

   four 
  oz. 
  salt-mouth 
  bottle 
  answers 
  well. 
  Povu'into 
  this 
  bottle 
  

   just 
  enough 
  ether-alcohol 
  (two 
  parts 
  sulphuric 
  ether 
  ; 
  one 
  part 
  

   absolute 
  alcohol) 
  to 
  cover 
  the 
  fragments. 
  I 
  find 
  that 
  these 
  

   proportions 
  give 
  better 
  results 
  than 
  equal 
  parts. 
  The 
  solution 
  

   is 
  easier 
  and 
  the 
  hardening 
  more 
  rapid. 
  The 
  ether-alcohol 
  

   should 
  be 
  added 
  until, 
  after 
  occasional 
  shaking 
  and 
  stirring, 
  

   no 
  fragments 
  remain 
  undissolved. 
  This 
  may 
  take 
  several 
  

   days. 
  It 
  should 
  finall}^ 
  possess 
  the 
  consistency 
  of 
  a 
  very 
  

   thick 
  oil. 
  The 
  solution 
  thus 
  obtained 
  may 
  be 
  labeled 
  no. 
  4. 
  

   No. 
  3 
  is 
  obtained 
  by 
  taking 
  two 
  volumes 
  of 
  no. 
  4 
  and 
  diluting 
  

   with 
  one 
  volume 
  of 
  ether-alcohol. 
  No. 
  2 
  by 
  proceeding 
  in 
  a 
  

  

  like 
  manner 
  with 
  no. 
  3. 
  N 
  

   and 
  sulphuric 
  ether 
  equal 
  parts. 
  

  

  fo 
  

  

  The 
  saturation 
  and 
  final 
  imbedding 
  is 
  accomplished 
  thus 
  : 
  

   ine 
  object 
  is 
  transferred 
  from 
  95 
  % 
  alcohol 
  to 
  solutions 
  i_, 
  2, 
  

   3»4, 
  successively, 
  in 
  each 
  of 
  which 
  it 
  remains 
  from 
  a 
  tew 
  

   hours 
  to 
  days, 
  depending 
  upon 
  the 
  size 
  and 
  permeability. 
  

   For 
  most 
  tissues 
  twenty-four 
  hours 
  in 
  each 
  will 
  suffice. 
  It 
  

   often 
  occurs 
  that 
  one 
  desires 
  merely 
  to 
  hold 
  the 
  object 
  in 
  situ 
  

   tor 
  cutting 
  ; 
  this 
  is 
  generally 
  attained 
  by 
  passing 
  the 
  object 
  

   through 
  solutions 
  2 
  and 
  4. 
  

  

  In 
  imbedding, 
  the 
  first 
  thing 
  necessary 
  is 
  to 
  provide 
  boxes. 
  

   A 
  hey 
  may 
  be 
  made 
  in 
  the 
  following 
  manner 
  :' 
  The 
  end 
  ot 
  a 
  

   pine 
  block 
  is 
  trimmed 
  to 
  the 
  desired 
  size, 
  e. 
  g., 
  i 
  cm. 
  long 
  

   by 
  1.5 
  cm. 
  wide. 
  For 
  a 
  box 
  of 
  these 
  dimensions, 
  and 
  i 
  cm. 
  

   ^eep, 
  a 
  piece 
  of 
  ordinary 
  porous 
  letter-paper 
  may 
  be 
  cut 
  m 
  

   rectangular 
  form 
  3 
  cm. 
  wide 
  by 
  6 
  cm. 
  long. 
  

  

  Place 
  the 
  center 
  of 
  the 
  end 
  of 
  the 
  block 
  over 
  the 
  center 
  

   01 
  the 
  paper, 
  the 
  longer 
  axes 
  of 
  the 
  end 
  of 
  the 
  block 
  and 
  pa- 
  

   P 
  er 
  parallel. 
  The 
  sides 
  are 
  now 
  pressed 
  against 
  thesides 
  ot 
  

   !he 
  block. 
  Then 
  the 
  ends 
  in 
  the 
  same 
  way, 
  leaving 
  lour 
  pro- 
  

   jecting 
  wings 
  at 
  the 
  angles 
  of 
  the 
  block. 
  Fold 
  in 
  the 
  wings 
  

   •"pmst 
  the 
  narrower 
  sides 
  and 
  press 
  the 
  ends 
  of 
  the 
  paper, 
  

   that 
  now 
  project 
  above 
  the 
  level 
  ofthe_box^_do^^ 
  

  

  =8^^S^i;^^;^,eh. 
  f, 
  Anat. 
  u. 
  Phys., 
  I 
  Abth., 
  p. 
  199. 
  1882) 
  uses 
  ^^<> 
  ^^^'Y'^^iu- 
  

   ^/;^Py, 
  the 
  other 
  much 
  thinaer. 
  Objects 
  are 
  transferred 
  f™"" 
  «^^°i^'^ 
  '^l^]"'' 
  £ 
  u^ 
  

   ZT 
  ''!''' 
  '« 
  ^Wcker. 
  Mlnot 
  (Whitman's 
  Embryologieal 
  M^thorls 
  P- 
  W. 
  l^ 
  

  

  ^f^e 
  solutions: 
  1. 
  Etherand 
  alcohol 
  equal 
  parts. 
  2. 
  Thi" 
  solution 
  of 
  cellm^in^ 
  J 
  

   Si! 
  ""^ 
  ^'^^^i^^^- 
  T 
  prefer 
  the 
  four 
  solutions 
  given 
  by 
  Apathy 
  (/e.t. 
  f. 
  wiss. 
  

  

  Mikr,, 
  p 
  

  

  ^^'faitman»s 
  Embryologieal 
  Methods, 
  p. 
  97. 
  18S5. 
  

  

  