﻿294 
  

  

  BOTANIC 
  AT- 
  GAZETTK. 
  

  

  [' 
  November, 
  

  

  ends 
  of 
  the 
  box. 
  A 
  'thin 
  plate 
  of 
  lead 
  is 
  placed 
  in 
  the 
  bot- 
  

   tom'* 
  and 
  the 
  imbedding 
  solution 
  poured 
  in. 
  The 
  object 
  is 
  

   . 
  1 
  — 
  c. 
  — 
  .1.. 
  __!...::_„ 
  and, 
  with 
  needles 
  wetted 
  in 
  

  

  Fine 
  needles 
  maybe 
  

  

  There 
  are 
  

  

  ^. 
  _ 
  the 
  method 
  

  

  en, 
  however, 
  to 
  be 
  the 
  best 
  suited 
  for 
  general 
  purposes. 
  

   Hardening 
  is 
  accomplished 
  b}' 
  various 
  methods^ 
  I 
  pi*e- 
  

   fer 
  that 
  given 
  by 
  Viallam,*' 
  of 
  immersing 
  in 
  chloroform 
  since 
  

   the 
  operations 
  may 
  be 
  carried 
  on 
  with 
  much 
  greater 
  rapidity. 
  

   An 
  air 
  tight 
  chamber 
  should 
  be 
  filled 
  with 
  chloroform 
  ; 
  a 
  very 
  

   wide-mouth 
  bottle 
  will 
  answer. 
  After 
  it 
  is 
  thoroughly 
  hard- 
  

   ened, 
  which 
  requires 
  about 
  twenty-four 
  hours, 
  the 
  mass 
  is 
  

   removed 
  and 
  the 
  paper 
  cut 
  from 
  the 
  sides. 
  

  

  It 
  is 
  now 
  read}' 
  to 
  fix 
  for 
  sectioning. 
  

  

  Basswood 
  blocks 
  

  

  Solution 
  na 
  

  

  are 
  trimmed 
  to 
  fit 
  the 
  clamp 
  of 
  the 
  microtome. 
  Solution 
  no. 
  

   3 
  is 
  poured 
  over 
  the 
  block 
  and 
  allowed 
  to 
  partially 
  harden; 
  

   pour 
  on 
  a 
  little 
  more 
  of 
  the 
  same 
  solution 
  and 
  press 
  down 
  the 
  

   cellojdin 
  block, 
  after 
  dipping 
  the 
  under 
  surface 
  in 
  ether 
  

   into 
  It. 
  Place 
  in 
  chloroform 
  until 
  hardened. 
  

  

  The 
  sections 
  are 
  now 
  cut. 
  

   knife 
  is 
  placed 
  

  

  -. 
  % 
  

   for 
  this 
  purpose 
  

  

  lur 
  uus 
  purpose, 
  or 
  one 
  can 
  easily 
  arrange 
  a 
  dripping 
  Jpi 
  

   ratus.^ 
  The 
  sections 
  may 
  be 
  removed, 
  with 
  a 
  camel's 
  h 
  

   brush, 
  to 
  a 
  watch 
  crystal 
  containing 
  an 
  alcoholic 
  stain. 
  I' 
  

   aqueous 
  stain, 
  they 
  are 
  passed 
  through 
  the 
  lower 
  grades 
  oi 
  

   alcohol, 
  50 
  %, 
  30 
  % 
  ^r. 
  „rot^.. 
  .^„;^^j 
  , 
  k..,^V 
  tliroucrh 
  tne 
  

  

  * 
  Suggested 
  by 
  Prof. 
  J. 
  E. 
  Reighard. 
  

  

  prefers 
  

  

  ,. 
  218, 
  18«) 
  

  

  iug 
  

  

  A 
  pi" 
  

  

  iasa«d 
  

  

  thrn,ll 
  ''^.'''P'"- 
  I" 
  the 
  cup 
  thus 
  formed 
  the 
  object 
  la 
  Imbedded. 
  - 
  i"" 
  "- 
  ,,^ 
  

   in 
  th. 
  h 
  ""T"' 
  °' 
  '"^^ 
  ""'^ 
  '''"'^ 
  '»'« 
  '^^ 
  bottom 
  of 
  the 
  cork. 
  The 
  mass 
  i^ 
  nf.J;^ 
  

   obhin^r 
  ^'h'"^ 
  ^"**^- 
  '^^'' 
  ^^^"^O'i 
  °fl""=^ 
  advantages, 
  since 
  hardening 
  and 
  fi^i"^ 
  

   ca^se 
  of 
  ,h 
  T 
  ''"' 
  '''^'- 
  ^°^'*-"^*^ 
  <^' 
  ^°rk, 
  which 
  gets 
  soft 
  and 
  spoils 
  the 
  alcoJoO^ 
  

   Zeulea 
  llZ^'t^'-' 
  "^^ 
  '^""'^^'^ 
  cylinders, 
  which 
  are 
  preferable 
  to 
  any* 
  J^^ 
  

   is 
  poured 
  ."h.'^K 
  If 
  '"• 
  '■ 
  '''''■ 
  ^^'^■•- 
  ^'^- 
  PP- 
  164-70, 
  1889) 
  uses 
  glass 
  boxe.. 
  u'o 
  

   Platl 
  Th 
  ""'^'^'^'^'"^ 
  «°'"''«n- 
  The 
  box 
  is 
  filled 
  to 
  the 
  brim 
  and 
  covered 
  w 
  tb 
  aj 
  

   ?hetial 
  K 
  ''T"'' 
  ''^'^ 
  ^"^^'^^^ 
  ^'^^ 
  hardening 
  and 
  allows 
  the 
  air 
  bubbles 
  to 
  e^^ 
  

   S'htWr"-'i'"'^'^"^°"^^^y«'belUar, 
  and 
  when 
  a 
  film 
  has 
  W<^' 
  i, 
  

   FlorLar 
  ;/' 
  ' 
  t°^°' 
  '^ 
  P*^^^"^ 
  °°- 
  I" 
  twenty-four 
  hours 
  the 
  mass 
  is 
  ready 
  fo 
  cu" 
  

   solutTon 
  a^r 
  rf/- 
  ^^>"°^™Pi-'^l 
  Soc. 
  Feb.. 
  1890) 
  imbeds 
  in 
  a 
  glass 
  capsuje 
  ^; 
  j„„, 
  

   Sy 
  SroLon 
  /?''''"°^^'"^^ 
  '"^^ 
  '""^ 
  evaporation 
  of 
  the 
  solvent. 
  Thoma 
  (J 
  _^ 
  

   thil 
  i 
  SeZ''"' 
  '.°"-' 
  P- 
  30i. 
  1S83) 
  covers 
  a 
  cork 
  with 
  a 
  thick 
  solution 
  of 
  cellmd 
  

  

  rtiallj 
  dry. 
  When 
  the 
  object 
  is 
  covered 
  it 
  is 
  immersed 
  in 
  alcohol 
  until 
  dry. 
  

  

  Recherches 
  sur 
  l-Hist. 
  des 
  Insectes,- 
  Paris, 
  1883. 
  quoted 
  in 
  Lee's 
  Vade 
  Mecu«. 
  

   See 
  Whitman's 
  Enthryological 
  Methods, 
  p. 
  115. 
  

  

  ^ 
  

  

  