﻿298 
  BOTANICAL 
  GAZETTE. 
  [ 
  November, 
  

  

  IS 
  more 
  uniformly 
  satisHictory. 
  The 
  tissue 
  is 
  allowed 
  to 
  re- 
  

   main 
  in 
  this 
  solution 
  about 
  12 
  hours. 
  It 
  is 
  then 
  taken 
  out 
  

   and 
  arranged 
  in 
  position 
  on 
  a 
  cork 
  or 
  block 
  of 
  wood 
  of 
  con- 
  

   venient 
  size 
  to 
  fit 
  in 
  the 
  jaws 
  of 
  the 
  microtome. 
  It 
  is 
  not 
  

   necessar}^ 
  that 
  the 
  corks 
  be 
  previously 
  soaked 
  in 
  collodion. 
  

   By 
  means 
  of 
  a 
  camel's 
  hair 
  brush 
  the 
  material 
  on 
  the 
  cork 
  is 
  

   covered 
  with 
  successive 
  layers 
  of 
  thick 
  collodion 
  until 
  it 
  is 
  

   quite 
  enclosed 
  in 
  the 
  mass, 
  allowing 
  each 
  coat 
  to 
  dry 
  slightly 
  

   before 
  applying 
  the 
  next. 
  After 
  the 
  tissue 
  is 
  covered 
  it 
  is 
  

   allow^ed 
  to 
  harden 
  in 
  the 
  air 
  a 
  few 
  minutes 
  and 
  then 
  placed 
  

  

  1 
  

  

  % 
  

  

  INIuch 
  difference 
  of 
  opm- 
  

  

  be 
  

  

  ion 
  exists 
  re^^arding 
  the 
  proper 
  strength 
  of 
  alcohol 
  to 
  use 
  for 
  

   hardening 
  the 
  collodion, 
  but 
  80 
  % 
  answers 
  very 
  \^'ell, 
  and 
  

   the 
  tissue 
  can 
  be 
  kept 
  in 
  it 
  a 
  long 
  time 
  without 
  deteriorating. 
  

   After 
  a 
  few 
  hours 
  the 
  collodion 
  will 
  be 
  hard 
  enough 
  to 
  sec- 
  

   tion. 
  For 
  sectioning 
  anv 
  sliding 
  microtome 
  will 
  answer 
  the 
  

   purpose, 
  but 
  one 
  especially 
  adapted 
  for 
  this 
  work 
  will 
  enable 
  

   one 
  to 
  incline 
  the 
  object 
  (which 
  can 
  be 
  clearly 
  seen 
  through 
  

   the 
  collodion) 
  in 
  any 
  desired 
  position 
  and 
  to 
  take 
  sections'" 
  

   any 
  desired 
  plane. 
  " 
  It 
  is 
  also 
  necessary 
  that 
  the 
  sections 
  

   removed 
  wdth 
  long 
  sweeping 
  cuts, 
  since 
  direct 
  cross 
  cutting 
  

   would 
  tear 
  them. 
  The 
  sections 
  should 
  be 
  kept 
  covered 
  wit}i 
  

   alcohol 
  while 
  being 
  removed, 
  and 
  then 
  floated 
  from 
  the 
  knite 
  

   to 
  the 
  slide. 
  The 
  slower 
  the 
  section 
  is 
  cut 
  the 
  better 
  it 
  W 
  

   usually 
  be. 
  Serial 
  sections 
  can 
  be 
  arranged 
  in 
  their 
  proper 
  

   place 
  on 
  the 
  slide. 
  For 
  fixing 
  the 
  sections 
  some 
  dry 
  ethe 
  

   ether 
  vapor 
  is 
  blown 
  upon 
  them. 
  A 
  very 
  convenient 
  fornioi 
  

   apparatus 
  for 
  the 
  purpose 
  can 
  be 
  made 
  by 
  fitting 
  two 
  tubej 
  

   into 
  a 
  wide-mouthed 
  bottle 
  as 
  in 
  making 
  a 
  wash-bottle, 
  excepj 
  

  

  ill 
  

  

  that 
  the 
  entrance-tube 
  dips 
  below 
  the 
  sm-face 
  of 
  the 
  ether 
  and 
  

   l!'L^:^,'^"*V^. 
  ^^ 
  ''^b^^-e 
  the 
  ether. 
  The 
  inner 
  end 
  of 
  the 
  exit- 
  

  

  apor 
  

  

  tube 
  should 
  be 
  expanded 
  into 
  a 
  bulb, 
  in 
  which 
  calcium 
  c 
  

  

  nde 
  IS 
  placed 
  to 
  dry 
  the 
  vapor 
  as 
  it 
  passes 
  out. 
  The 
  ether 
  vaP^' 
  

   d 
  issolvesthe 
  collodion 
  and 
  fastens 
  the 
  sections 
  to 
  the 
  si 
  • 
  

   ihe 
  sections 
  are 
  then 
  washed 
  with 
  water, 
  stained, 
  the 
  " 
  

   P 
  us 
  stain 
  washed 
  off 
  with 
  water, 
  the 
  sections 
  dehydrated 
  ^'^ 
  

   alcohol, 
  cleared 
  and 
  mounted 
  in 
  balsam. 
  For 
  cleanng. 
  

  

  found? 
  ""'"1 
  ^>^ 
  P™^- 
  Gage 
  for 
  animal 
  histology 
  w" 
  , 
  

   found 
  to 
  work 
  admirably 
  It 
  consists 
  of 
  3 
  parts 
  of 
  turp 
  n^^ 
  

  

  the 
  ^ 
  .T^^^^' 
  '^^'^- 
  It 
  clears 
  quickl/and 
  will 
  not 
  n 
  

   which^if^^;.! 
  l^f-- 
  , 
  For 
  stSning,^ 
  one 
  ...t 
  -e 
  d 
  , 
  

  

  s 
  

  

  men 
  seems 
  best 
  adapted 
  for 
  his 
  purpose, 
  but 
  for 
  g^ 
  

   Sn^'^"'''^-^'^'^^^ 
  «^^"^« 
  especially 
  adapted 
  to 
  coHod' 
  

  

  Some 
  difficulty 
  may 
  arise 
  in 
  cutting 
  sections 
  which 
  ha^'^ 
  

  

  