8 FOOT. [Vor. XII. 
substances may be fluid. (The polar rings in the egg of 
Clepsine consist, according to Whitman, of “a transparent 
fluid substance.) The granular appearance of the archoplasm 
is most marked at the points of greatest aggregation; that 
part aggregated at the poles as polar rings appearing more or 
less granular with all fixatives. 
Fig. 7 shows a typical example of the presence of the archo- 
plasm within the spindle and at its poles!. 
In some cases (possibly caused by the action of the fixative) 
the archoplasm is not equally distributed in the spindle, but is 
aggregated at its edges and around the chromosomes, leaving 
areas entirely free from the substance; again, it is aggregated 
in longitudinal lines, which appear as heavy blue fibres among 
the red anastomosing spindle fibres. At the poles of the spindle, 
we see the red cytoplasmic rays of the attraction-spheres and a 
pronounced aggregation of the archoplasm. This egg (Fig. 7) 
was killed in Merkel’s fluid, which thus far has proved relatively 
unfavorable for chromosomes and centrosomes.” 
Fig. 8 shows a cross-section through the fertilization cone 
(the structure which appears to be identical with the “cone of 
attraction’ of other animal forms).® The archoplasm is aggre- 
gated around the sperm, and the part in contact with the 
sperm appears to be blended with the red cytoplasm, forming 
a homogeneous mass, suggesting a fluid condition of both 
substances. This figure gives us a typical illustration of the 
action of corrosive acetic upon the archoplasm, causing it to 
form into masses, as mentioned above. 
1 This phase of the archoplasm recalls Strasburger’s ‘‘Kinoplasm”; Flem- 
ming’s “ Zelle.” Merkel und Bonnet’s Ergebn. der Anat. u. Entwickg., Bd. 
III, 1893, pp. 66, 104. 
* The lithographer has not indicated the faint chromosomes shown in the 
original sketch. For the shape of the chromosomes of this spindle see my “ Pre- 
liminary Note on the Maturation and Fertilization of the Egg of AJl/lolobophora 
foetida.” JOURNAL OF MORPHOLOGY, vol. IX, p. 94. 
8 For further details as to the cone and the fertilization of this egg, see my 
“ Preliminary Note on the Maturation and Fertilization of the Egg of A//olobophora 
foetida,” JOURNAL OF MORPHOLOGY, vol. IX, 1894. The “sperm granules ” give 
a staining reaction similar to the nucleoli; with aurantia and iron haematoxylin, 
the nucleoli of the pronuclei, the nucleoli outside the first maturation spindle, and 
the sperm granules, all stain yellow, while the chromosomes and sperm stain blue. 
