No.1.] BUDDING IN GOODSIRIA AND PEROPHORA. I51 
alcohol, I was limited to these for the preservation of my 
specimens. Luckily, however, the former proved to be a 
favorable medium for the purpose. Some of my material has 
proved to be excellently well preserved, better than any I have 
been able to get of some other Ascidians by using a large 
variety of reagents. Some specimens preserved in absolute 
alcohol were found to be valuable as collateral material. 
No opportunity has been afforded me for studying living 
specimens with any detail, but they are much too dense and 
opaque to permit of being very satisfactorily studied in this 
condition. Likewise, since the species belongs to that cate- 
gory of Ascidians in which the mantle clings closely to the 
test, it is impossible to free the zooids from the colony in 
preserved specimens so as to study them whole with much 
satisfaction. One is consequently obliged to depend on the 
study of small colonies, or small pieces of colonies cleared in 
oil; on dissections; and on thin sections. The first mentioned 
method is particularly valuable. 
Of the numerous stains employed I have found Paul Mayer’s 
Hzemalum, and Grenacher’s Alum Carmine to give the most 
satisfactory results. 
2. DESCRIPTION OF THE SPECIES. 
As we are dealing with a new form, it will be necessary to 
describe it; and this is the more incumbent because it belongs 
to a group of Ascidians not very well known to science. 
As mentioned in my preliminary paper ('94), it belongs to the 
Polystyelidae, a family founded by Herdman (86). The author 
has given a good historical résumé of our knowledge of the 
group, and I may consequently touch this phase of the subject 
lightly. The six genera of which the family is at present 
composed were, with one exception, all established between 
1843 and the time of Herdman’s description of the Challenger 
collection in 1886. This one exception was added by the 
author himself. They were described by different writers and 
assigned by them to different larger groups already known; 
Carus (43), for example, regarding the genus described by him 
