160 LATTER. [VoL. XII. 
were true it would be impossible to explain ‘la production 
d’etoiles multiples et distantes dans le cormus d’un Botryllien.” 
The remoteness of the young buds from any older zooids in 
Goodsiria has likewise frequently proved a stumbling-block to 
me in seeing how they could in such cases have been produced 
in the usual way, z.c. from the wall of the peribranchial sac. 
But I have given much attention to the point, and am quite 
convinced that in reality this is their only source. Herdman 
(g6) expresses the opinion that the ampullae of the vessels will 
be found to give origin to the buds, but such is apparently 
not the case here any more than it is in Botryllus. 
The frequent remoteness of the buds from their parents 
must be due to their having grown away from the latter before 
they become fully severed from them. From the firmness of 
the test and the character of the young buds I can hardly 
believe that they have any power of independent migration 
throughout the test. 
This conjecture, that the remoteness of the buds from their 
parents is due to the growth of the bud before it becomes 
severed, harmonizes with the view that pallial budding as it 
takes place in Botryllus and Goodsiria is not of necessity 
fundamentally different from the stolonic method of budding. 
The comparison between these two methods becomes still 
more interesting from the discovery that in Pevophora the 
buds are connected with the septum of the stolon by their 
peribranchial instead of their branchial sacs. But I shall 
discuss this point further after having described in detail the 
development of the bud in each species. 
There is certainly no septum in the vessels of the test, 
(Bigs. 3, 5.7, ete. oki. XU ec:ves.); and at budsawerestojbe 
produced in connection with them, it would have to be by a 
method essentially different from any of the generally recog- 
nized types of gemmation among Tunicates. Since the blood 
of the zooids passes freely into the testicular vessels, and since 
the young sexual cells float in the blood, it is not beyond the 
range of possibility that these cells may pass into the ampullae 
of the vessels, and there form themselves into the “inner 
vesicle,” the all important precursor of the blastozooid. 
| 
' 
‘ 
| 

