No.1.) BUDDING IN GOODSIRIA AND PEROPHORA. 161 
It is an interesting fact that Herdman ('86, p. 90 e¢ seq.) has 
observed a process in Colella pedunculata which would appear 
to be a realization of that here imagined. As the author 
himself says, his observations are rather fragmentary, and 
consequently his account is much less full than we might wish 
it £6; be: 
That the buds arise in this anomalous manner in this 
genus, he, however, seems to be convinced, and his descrip- 
tion and figures undoubtedly furnish good ground for his 
conviction. It is certainly very much to be hoped that oppor- 
tunity will before long be afforded some zodlogist to study 
the subject more fully. In a few instances I have found a 
massing of cells within the ampullae that is strongly sugges- 
tive of the process described by Herdman. Minute examination 
of these aggregates has, however, failed to furnish any evidence 
that they produce buds. Two such cases are shown in Fig. 7, 
Pl. XII. They are quite conspicuous when cleared in oil and 
examined with a low magnification. 
The vessels generally occupy a deeper position in the test 
than do the zooids, so that the test surrounding the zooids is 
less thickly penetrated with them than are its deeper portions. 
This is shown in Fig. 5, Pl. XII. 
It has been mentioned above that the buds become fully 
severed from the parent zooids at an early stage in development. 
This fact raises the question of the extent to which the zooids 
in this species are independent of one another. It is certain 
that many of them are in connection with the vessels of the 
test for at least a portion of their lives, and are consequently 
in vital connection with one another. But this connection is 
almost certainly secondary, and, I believe, not essential to the 
development of the zooids. 
As the young buds become larger, they press more and more 
closely against the vessels that were in contact with them, or 
nearly so, at the beginning, and by this pressure a fusion of 
the vessel wall with the outer or ectodermal wall of the bud 
is produced, and then later a perforation of the fused walls 
occurs, and the lumen of the vessel is thus brought into com- 
munication with the body space of the zooid. 
