No.1.] BUDDING IN GOODSIRIA AND PEROPHORA. 183 
and Fig. 48, Pl. XVI, are drawn, are particularly to the point, 
and these I have consequently selected to describe. Figs. 45— 
47 represent transverse sections, in order from before back- 
ward, of a bud slightly less advanced than the one in nearly 
longitudinal section shown in Fig. 48. The sections are 
considerably oblique, so that the one, Fig. 44, passing through 
the mouth of the duct makes it appear to be inclined to 
one side. The ganglion is not touched by this section, my 
purpose in figuring it being to show that the hypophysis mouth 
exists at this time, though very small. This section, with the 
following one, not reproduced, leaves no doubt on the point. 
Figs. 45 and 46 are from sections a little farther back. They 
need very little explanation. They show the thickened ventral 
wall of the duct, which is (particularly in Fig. 46, six sections 
farther back toward the place where the duct is still unsevered 
from the vesicle) not distinguishably separated from the wall 
of the vesicle. In this thickened region, Fig. 46, cell division 
is more frequently seen than in other parts of the tissues. 
Fig. 47, one section farther back, shows that the duct still 
communicates by a wide opening with the primitive vesicle. 
Three more sections take us beyond the duct entirely, so it is 
obvious that the communication shown in Fig. 47 is at the 
posterior part of the duct. If now we turn to the longitudinal 
sections, one of which is shown in Fig. 48, Pl. XVI, we find 
that the lumen of the duct no longer communicates with the 
vesicle posteriorly, but that there exists a trace, ~e¢.0., of the 
opening by which this communication was earlier effected. 
But the most important fact to be learned from this section is 
that anteriorly the ganglion, g/., has now become separated 
both from the ventral wall of the duct and from the wall of the 
vesicle ; but that posteriorly, where the duct has not yet fully 
severed its connection with the vesicle, the ganglion is still 
confluent with the large cell mass that forms at the same 
time the ventral wall of the duct and the dorsal wall of the 
vesicle. 
We are thus led to recognize that szmultaneously with the 
closing off from the tnner vesicle from before backward of the 
hypophyseal duct, the ganglion becomes differentiated in the same 
