188 RILTER. PVioL. xa: 
any groups higher than species. To some extent this radical 
departure receives support from Perophora annectens. But I 
fully agree with Herdman and Seeliger in thinking that the 
system adopted by Lahille, at any rate as regards his families 
and suborders, comes no nearer being a natural one than the 
other systems which it is intended to supplant. But it would 
be outside the purpose of the present paper to enter upon a 
general discussion of tunicate classification. 
In view of the apparent hesitation of some students of the 
Tunicata, with whom I have had personal conversations, to 
accept my conclusion that P.aunectens really does include 
such widely varying forms, I had thought to present in the 
present publication a more detailed and fully illustrated de- 
scription of all the transitional forms than is contained in my 
former paper. However, further reflection has made it seem 
to me that such a discussion will belong more appropriately to 
a comprehensive treatment of all our Pacific Coast species of 
the genus. Such a treatment will be contained in a mono- 
graph of the California Tunicata which is now in course of 
preparation by myself and one of my students, Mr. F. W. 
Bancroft. 
As stated in my preliminary, my results on the budding of 
Perophora have nearly all been obtained from the study of P. 
annectens. From the abundance of this species in Monterey 
Bay, Cal., and from the compactness of the colonies, it is 
very easy to get unlimited material, and whenever I have 
collected it, the buds in all stages of development have been 
present in great numbers. /. Lzstevz, on the other hand, at 
least in the Bay of Naples, I found to present almost insup- 
erable difficulties in the way of procuring buds in sufficient 
quantities to enable me to get the necessary stages, and those 
in such numbers as to make a satisfactory study of their 
development. Both the zooids and the stolons are very delicate, 
and they cling so closely to the stones on which alone I found 
them that the young buds are removed with very great diffi- 
culty. I succeeded, however, in securing specimens enough 
to-enable' me to make sure that there is no ditference “of 
moment between the bud-development in the two species. 
