NOmZz ih Lie wE GG, Of AMITA AND TES. {LEA VAGE. 325 
which the pair were still engaged in spawning. Eggs were 
taken from the nest and at once placed under observation, and 
the following record made: Three sets of eggs were put in 
glass dishes, and Mr. Nomura, Mr. Eycleshymer, and myself 
observed independently the segmentation of these fresh-laid 
eggs. The first segmentation groove began at 5.30 p.m. The 
second meridional began at 6.30 in those eggs which were 
placed in the sun after the beginning of the first furrow, and 
at 7 o'clock in those eggs kept in the shade out of the 
sun’s rays. 
“The third and fourth meridional furrows began simulta- 
neously at 7.30. The first equatorial to be seen made its 
appearance at 8.25, and completed the circuit at 8.40. The 
next set of eight peripheral meridional furrows began at g 
o'clock, and the second apparent equatorial furrow began at 
9.30. 
‘«« Amiae are to be found in Oconomowoc Lake during the win- 
ter months, living in schools closely huddled together in the 
bottom of pockets or shallow depressions of the gravelly bed of 
the lake, among the water weeds. These fish may be speared 
from a boat, or, if the lake is frozen over, through holes in the 
ice. They lie so close together that occasionally two individ- 
uals are impaled on the fish spear by one throw. When thus 
disturbed they scatter from their resting-place, moving out a 
short distance, to return quickly after the first few disturb- 
ances. When alarmed by repeated thrusts of the spear, they 
remain away for hours at a time. Why they should be so tena- 
cious of these resting-places will probably be explained when 
we know why they choose them in the first place. It is pos- 
sible that the temperature of the water is higher in these places 
than elsewhere, owing to the entrance of spring streams into 
the lake bottom.” 
3. Some Further Data. 
In some of the eggs taken from this nest, and kept under 
close observation, the first indication of the first cleavage ap- 
peared at the upper pole at 7.54, z.c., 2 hrs. 24 min. from the 
supposed time of deposition. At this time the first cleavage 
