130 MUNSON. [Vol. XV. 



epithelium, previously distinctly and uniformly outlined, now 

 becomes obscure and ill-defined ; the fibers of the cell body 

 become more distinct and fewer in number; cell boundaries 

 seem to become very indistinct, and large, open, non-stainable 

 spaces appear to occupy the larger portion of the former cell. 

 The remaining fibers appear packed along one side of the 

 former cell, and in this remnant of protoplasm the somewhat 

 obscured nucleus lies imbedded. The whole epithelium in sec- 

 tion now has a very irregular, ragged outline. 



Owing to these changes in the epithelial cells, the same 

 method of preservation has different effects, not only on the 

 cytoplasm of the cell body, but also on the nuclei. In well- 

 preserved conditions of the cell, previous to the considerable 

 accumulation of granules, the chromatin consists of deeply 

 stainable spheres arranged in a circle around the periphery of 

 the spherical, vesicular nucleus, forming in haematoxylin stains 

 a dark beaded ring around a clear central area. At times, but 

 not always, a dark body more minute than the chromatin gran- 

 ules can be seen to occupy the center of this clear central 

 area. 



After the accumulation of the granules in the cytoplasm, 

 however, this expanded spherical condition of the nucleus and 

 the regular peripheral arrangement of the chromatin is not to 

 be observed. The nucleus seems to collapse, become oval, and 

 the distribution of the chromatin becomes irregular. The chro- 

 matin bodies themselves, losing their uniformity in size and 

 their regular spherical form, appear as if broken up. They may 

 also become aggregated into a more or less homogeneous mass. 

 This latter condition of the nuclei is often to be observed in 

 those cells of the egg stalk which show evidence of gradual 

 disintegration. Since the other forms of the nucleus are found 

 in their immediate vicinity, this peculiarity cannot be attributed 

 to the reagents used. Certain methods of killing, however, as, 

 for example, Ehrlich's bichromate, when allowed to act for 

 several days, give to the chromatin of all the nuclei an appear- 

 ance resembling this latter form. 



5. Forviation of follicles. — The pressure caused by the 

 tonicity of the muscle fibers is greatest between the follicular 



