1 50 MUNSON. [Vol. XV. 



ance of a true follicle epithelium. At times this layer of cells 

 may not extend to the distal pole ; and in still other cases 

 several layers may appear at various points. 



These enveloping cells appear to be continuous with the epi- 

 thelial cells of the stalk, but their boundaries are more sharply 

 defined. The nuclei of these cells resemble the nuclei of the 

 germinative epithelium, but their cytoplasm is always packed 

 with stainable granules resembling yolk granules. The nuclei 

 within the egg present every similarity to the nuclei of 

 these surrounding cells ; and, like the latter, in advanced 

 stages of metamorphosis of the egg they are surrounded 

 by deeply staining granular areas of protoplasm, indicating 

 cell outlines. Often, however, the nuclei are seen imbedded 

 in interwoven strands of protoplasm, where no cell boundaries 

 are visible. This may occur in different portions of the same 

 egg. On their first appearance the nuclei are often uniformly 

 distributed throughout the yolk, in which cases the yolk may 

 be normal, or else slightly broken up into comparatively large 

 masses, giving a vague suggestion of cleavage. 



In stages farther advanced the nuclei, which at first showed 

 no indication of cell boundaries, become more or less grouped 

 into patches. The yolk granules, previously evenly distributed 

 throughout the egg, evidently disappear in patches at different 

 times, till one pole of the egg may be nearly devoid of yolk 

 granules. It then shows only the strands of protoplasm with 

 scattered granules, and nuclei imbedded in them ; while the 

 other pole may still have the normal appearance, with the 

 exception of here and there an isolated nucleus. 



In section, except in the earliest phases of metamorphosis, 

 the outlines of these eggs become irregular (PL XIV, Fig. 31). 

 The egg membrane becomes indented, folded, and perforated 

 in various ways. The perforations may pass transversely or 

 obliquely, and in these perforations cells resembling the 

 granular cells surrounding the egg are often observed. These 

 perforations often communicate with spaces between the outer 

 tunic and the infolded egg membrane, which spaces may be 

 filled with granular cells resembling those observed in the 

 perforations. 



