x\o. 2.] THE OVARIAN EGG OF LIMULUS. 187 



XV, Fig. 89). This zone of granules is often not so definitely 

 outlined as the central body, but its outer border is often ser- 

 rated. It is, however, sharply differentiated from the surround- 

 ing cytoplasm. From this dark-brown body radial fibers extend 

 into the yellow cytoplasm surrounding it. In Ehrlich's haema- 

 toxylin, followed with eosin, the body may show a blue center 

 composed of granules. This is then surrounded by a red zone 

 of concentrically wound fibers more or less interwoven ; and 

 from this again red fibers can be seen to radiate through- 

 out the cytoplasm ; and in some sections of the latter, where 

 granules are less numerous, the radial fibers can be traced to 

 the very periphery of the egg (PI. XIV, Fig. 60 ; PL XV, Fig. 

 67). As the egg grows the body increases in size, and the 

 form described above may thus become relatively large and 

 conspicuous. Stained in erythrosin and cyanin, such a large 

 body may be seen to consist of a large central, spherical mass 

 of granules with a blue tint. This is again surrounded by a 

 comparatively thick zone of bright-red fibers arranged concen- 

 trically around the central granules. And around this again 

 can be seen a radial arrangement of the cytomicrosomes (PI. 



XVI, Fig. loi). 



In Ehrlich's haematoxylin and acid fuchsin the body may 

 appear as a conspicuous deep red, rather small, definite, refrac- 

 tive body in the center of a zone of blue granules (PI. XV, 

 Figs. 6Z, 82). The cytoplasm having a less intense red colora- 

 tion, this form of the body is often a conspicuous and beauti- 

 ful preparation. From this as a center, also, radial fibers can 

 often be distinctly traced to the periphery of the egg. Many 

 of the red radial fibers can be seen to penetrate the blue zone of 

 granules, and to proceed directly from the red central body 

 (PI. XV, Fig. 68). At other times the blue granules of the 

 zone are so abundant that the radial fibers cannot be observed 

 in it, and they consequently appear to end at the periphery of 

 the blue zone (Fig. 6"]). The round, red central body is occa- 

 sionally seen to be surrounded by a clear space which again is 

 bound by a definite red staining wall, around which the blue 

 zone of granules is arranged (Figs. 67, 82). The central body 

 may be very minute ; and radial fibers, and, as it often appears. 



