No. 2.] THE OVARIAN EGG OF LIMULUS. 189 



zone of considerable thickness, and having the form of a horse- 

 shoe (PI. XV, Fig. 76). At the upper part of this fibrous 

 protoplasm are imbedded several aggregations of blue granules ; 

 and, surrounding the whole, and slightly removed from it, in 

 the red cytoplasm, is seen an irregular circle of these blue 

 granules like a wreath, which, at the opening of the horseshoe- 

 shaped protoplasm, forms an irregular mass of the blue 

 granules. When stained in Ehrlich's haematoxylin alone, the 

 body appears as an unstained spherical mass of fibrous proto- 

 plasm of considerable size, and definitely limited, around which 

 are arranged, in a radial manner, numerous deep-blue granules 

 apparently associated with a radial system of fibers proceeding 

 from the body as a center (PI. XVI, Fig. 96). Within such a 

 body similar blue granules are found distributed, but most 

 frequently aggregated into groups occupying vacuoles which 

 with the blue granules appear like nuclei. Again it may appear 

 as a conspicuous spherical body of the same fibrous protoplasm, 

 which stains a deep red in erythrosin or acid fuchsin. It may 

 enclose one or two nuclei-like bodies surrounded by a denser 

 zone of the same fibrous protoplasm (PL XV, Figs. 64, 86). 

 If the acid fuchsin be preceded with Ehrlich's haematoxylin, 

 the central body (or bodies) is seen to contain the same blue 

 granules resembling chromatin granules of nuclei (Figs. 86, 88). 

 There may be several of these central bodies containing blue 

 granules scattered irregularly between the fibers of a large felt- 

 work of delicate protoplasmic fibers, as seen in PI. XVI, Fig. 95. 

 Occasionally the blue granules appear as highly refractive 

 bodies lying between the fibers, that may have a comparatively 

 regularly concentric arrangement (PI. XIV, Fig. 61; PI. XV, 

 Fig. 72). Occasionally none of the granules can be observed 

 (Fig. 69), but this may be due to the staining. Erythrosin, for 

 instance, makes the whole body very conspicuous, but does not 

 always differentiate the internal structures. The whole body 

 may be relatively large, oval in form, and homogeneous in 

 structure, having in this case, as in most cases, a system of 

 surrounding radiations that extend far into the cytoplasm (PI. 

 XVI, Fig. 99). It may at times have the form of a spindle, 

 with its longitudinal axis not exceeding the transverse axis, the 



