THE OVARIAN EGG OF LIMULUS. 21 7 



three vacuoles filled with blue granules. The entire body is surrounded by a zone 

 of blue granules, which are more numerous at the opening of the horseshoe-shaped 

 archoplasm. Method : Merkel's fluid, Ehrlich's haematoxylin, and eosin. 



Fig. 77. Section showing peripheral bodies, a central sphere with astral rays. 

 The center of the sphere consists of closely packed blue granules, and this is 

 surrounded by a thick dense red limiting membrane. Method : Merkel's fluid, 

 erythrosin, and cyanin. 



Fig. 78. Section showing a proximal polar mitosome, a conspicuous vitelline- 

 body, staining red, and surrounded by a zone of blue granules. Similar granules 

 are seen also at the pole opposite the stalk of the egg. 



Fig. 79. Section of egg showing germinal vesicle with a prominent " Haupt- 

 nucleolus," and an extruded " Nebennucleolus "; peripheral bodies at the periphery 

 of the egg ; in the cytoplasm, an archoplasmic sphere, containing two central 

 structures. Method : Merkel's fluid, safranin, and Lyon's blue. The sphere 

 alone, blue or greenish ; everything else, red. A deep-red circle at the proximal 

 pole. 



Fig. 80. Section showing a vitelline-body, consisting of a central, irregular, 

 granular mass, from which radiate straight fibers, which at a certain distance from 

 the central body are again limited by the granules of the cytoplasm. A peculiarly 

 modified fibrous protoplasm exists in the neighborhood of the body, and this is 

 connected with three small refractive bodies imbedded in a strand of protoplasm. 

 Method : Merkel's fluid, Biondi-Ehrlich. 



Fig. 81. Section showing germinal vesicle with vacuolated nucleolus; a vitel- 

 line-body or sphere, consisting of fibrous protoplasm, containing two central 

 granular bodies. Method : Merkel's fluid, erythrosin, and cyanin. 



Fig. 82. Section showing germinal vesicle, nucleolus ; in the cytoplasm a 

 large, sharply defined, deep-red centrosome, surrounded by two zones of archo- 

 plasm and astral rays, the latter on one side being modified into a conspicuous 

 polar mitosome. Method : Merkel's fluid, haematoxylin, and acid fuchsin. 



Fig. 83. Section showing germinal vesicle with vacuolated nucleolus ; in the 

 cytoplasm a large, homogeneous sphere containing vacuoles with blue granules, 

 partly surrounded by groups of blue granules resembling nuclei. Method : Merkel's 

 fluid, Ehrlich's haematoxylin. The homogeneous part of sphere, unstained. 



Fig. 84. Section showing germinal vesicle, a vacuolated nucleolus ; in the 

 cytoplasm a large oval fibrous vitelline-body, containing granules, and connected 

 with the periphery of the egg by a modified fibrous protoplasm resembling that of 

 the vitelline-body. Method : Merkel's fluid, Biondi-Ehrlich stain. 



Fig. 85. Section showing germinal vesicle with diverticula ; a large vesicular 

 nucleolus, containing granules, and in the cytoplasm a large, finely granular sphere, 

 which is partly surrounded by a dense zone of granular archoplasm. Method : 

 corrosive-acetic, haematoxylin, and picric acid. 



Fig. 86. Section showing a conspicuous, deep-red, fibrous vitelline-body 

 (sphere), containing two central vacuoles with blue granules, and numerous 

 peripheral vacuoles with similar blue granules, the whole being surrounded by a 

 zone of larger metaplasmic granules and astral radiations. Method : Merkel's 

 fluid, Ehrlich's haematoxylin, acid fuchsin. 



Fig. 87. Section showing germinal vesicle, a vacuolated nucleolus, and in the 

 cytoplasm one or two centrosomes surrounded by conspicuous silken astral rays 

 that are not sharply limited. Method : corrosive-acetic, haematoxylin. 



