THE OVARIAN EGG OE LIMULUS. 



EXPLANATION OF PLATE XVL 



219 



Fig. 91. Section showing germinal vesicle, nucleolus ; in the cytoplasm a 

 granular yolk-nucleus at the proximal pole ; connected with it, a proximal polar 

 mitosome. Method : Merkel's fluid, Biondi-Ehrlich. 



Fig. 92. Section showing germinal vesicle ; in the cytoplasm a conspicuous 

 aster with a large, deeply staining granular centrosome. Method : Merkel's fluid, 

 erythrosin, and cyanin. 



Fig. 93. Section showing germinal vesicle with nucleolus ; peripheral bodies 

 at the boundary of the egg ; a peculiar vitelline-body consisting of a spherical mass 

 of fibrous protoplasm, connected by a stalk with a finely striated protoplasm at 

 the pole opposite the stalk of the egg ; a few refractive granules, near the stalk of 

 the spherical body. 



Fig. 94. Section showing germinal vesicle with a large vacuolated nucleolus ; 

 in the cytoplasm a large homogeneous fibrous vitelline-body, having a mass of 

 granules at, one pole. 



Fig. 95. Section showing germinal vesicle with diverticulum, and a conspicu- 

 ous nucleolus ; a large vitelline-body, having numerous vacuoles, containing the 

 blue granules and a central structure, probably the centrosome. Method : Merkel's 

 fluid, erythrosin, and cyanin. 



FiO. 96. Section showing vitelline-body with astral rays, and surrounded by 

 groups of blue granules arranged radially; within the body, also, a group of blue 

 granules. The fibrous portion unstained. Method : Merkel's fluid, Ehrlich's 

 haemato.\ylin. 



Fig. 97. Section showing vitelline-body as a large granular central body, sur- 

 rounded by a zone of archoplasm and a conspicuous system of radial fibers, 

 which extend to the periphery of the egg, and is especially pronounced at the pole 

 opposite the point of attachment of the egg. Method as above. 



Fig. 98. Section showing vitelline-body with several granular central areas. 

 Method as above. 



Fig. 99. Section showing germinal vesicle with a large vacuolated nucleolus ; 

 in the cytoplasm a large, homogeneous sphere staining deeply, and surrounded by 

 radial fibers. Method : Merkel's fluid, erythrosin, and cyanin. 



Fig. 100. Section showing germinal vesicle, containing vacuolated nucleolus ; 

 in the cytoplasm a distinct sphere, containing a central granule, centrosome, with 

 radial fibers and granules, which is again bounded by layers of fibrous protoplasm 

 concentrically arranged ; the body, surrounded by a zone of large, stainable gran- 

 ules ; on the proximal side a modified polar mitosome. Method : Merkel's fluid, 

 Biondi-Ehrlich. 



Fig. ioi. Section showing central sphere, consisting of a large, spherical, cen- 

 tral body staining blue, and compoi;ed of blue granules, and another zone of 

 concentrically arranged fibrous protoplasm ; indications of astral radiations. 

 Method: Merkel's fluid, Ehrlich's haematoxylin, and acid fuchsin. 



Fig. 102. .Section showing germinal vesicle with large central nucleolus ; in 

 the cytoplasm a conspicuous sphere, consisting of large granules, a central granu- 

 lar body being separated from an outer granular zone by a light ring nearly free 

 from granules. Method : Merkel's fluid and Biondi-Ehrlich stain. 



Fig. 103. Section showing germinal vesicle with a large nucleolus. At one 

 pole of the germinal vesicle in the cytoplasm there is an area which shows a faint 



