2 20 M UN SON. 



radial striation and also a faint concentric striation. It is less granular than the 

 rest of the cytoplasm, and is no doubt the sphere. It resembles the condition 

 seen in Fig. 104, but is less conspicuous. 



Fig. 104. Section drawn with a camera with the same magnifying power as 

 the preceding figures, and showing the sphere in connection with the germinal 

 vesicle. The latter contains a large nucleolus. The hyaline protoplasm, consti- 

 tuting the " Kernpol " area, is seen near the point of attachment of the egg. 

 Method : platinum chloride, erythrosin, and cyanin. 



Fig. 105. Germinal vesicle with diverticula containing the nuclear network 

 and " Nebennucleoli "; also a large " Hauptnucleolus," showing an opening into 

 the central cavity. 



Figs. 106, 108, 115-11S. Nucleoli taken from the living egg of an animal 

 thirteen inches long. In Figs. 115, 116 two " Hauptnucleoli," one large and one 

 small, are present. In Fig. liS the two are closely united. 



Fig. 107. Germinal vesicle with a nucleolus containing within it a network 

 resembUng that of the germinal vesicle. 



Fig. 109. Section showing a condition of the sphere similar to Fig. 104, cut 

 at right angles to the principal egg axis. Method : same as above. 



Fig. no. Germinal vesicle containing a "Hauptnucleolus," from which a 

 " Nebennucleolus " has been extruded. 



Fig. III. Germinal vesicle containing a nucleolus with the " Nebennucleolus " 

 partly extruded. 



Fig. 112. Germinal vesicle with a " Nebennucleolus" partly extruded. In one 

 diverticulum of the germinal vesicle is seen a pale " Nebennucleolus," and in the 

 cytoplasm near by are two similar bodies. 



Fig. 113. Germinal vesicle with a " Hauptnucleolus," from which a " Neben- 

 nucleolus " is partly extruded. 



Fig. 114. Section showing the germinal vesicle containing a large nucleolus ; 

 and in the cytoplasm a large sphere, in which a radial striation can be made out. 

 The center contains a large clear area, in which a centrosome is difficult to find, 

 when the other portions of the sphere are made prominent. In the present figure 

 the central body with its centrosome and archoplasmic zone was taken from 

 another section of an egg of exactly the same size as the present one, as in that 

 case the central portion was better preserved than the peripheral portion. 

 Method : platinum chloride and Biondi-Ehrlich stain. Excellently preserved. 



