286 MONTGOMERY. [Vol. XV. 



or so) of globules of varying size which appear to lie loose 

 within the germinal vesicle. An intravesicular network is 

 sometimes present, and serves to unite the granules of the 

 macula. ... It is possible that the homogeneous matrix 

 above described may represent the remains of such a network, 

 the filaments of which have shrunk up into a mass on contact 

 with the hardening reagent " (picric acid and alcohol). 



Trinchese ('80, according to Platner, '86) found in the germinal 

 vesicle of Aniphoriiia cocrulea a " macchia germinativa laterale 

 ^ accessoria," and a "macchia germinativa principale," the 

 latter being about seven times the size of the former. 



Balbiani ('81) investigated the salivary gland cells of the 

 Chironomus larva. There are here "Deux gros nucleoles irre- 

 guliers, larges de 0.03 a 0.04 mm., bosseles a leur surface, et 

 formes d'une substance refringente granuleuse, creusee d'un 

 plus ou moins grand nombre de vacuoles isolees ou confluentes. 

 II arrive assez souvent que les deu.x nucleoles se confondent par 

 une partie plus etroite qui les reunit comme une sorte de pont ; 

 d'autres fois enfin, ils se fusionnent plus ou moins intimement 

 en un seu! nucleole, dont le diametre est le double de celui des 

 nucleoles isoles." The ends of the chromatin filament are 

 apposed against the nucleolus ; and the latter differs both 

 chemically and morphologically from this "cordon nucleaire." 



Giard ('81) observed in the egg of a Spionid during life a 

 single central nucleolus. A certain time before completed 

 maturation an " element cellulaire " appears in the nucleus, 

 which is a little smaller than the latter, and encloses in its 

 center a small "noyau": " D'abord fort eloigne du nucleole, il 

 s'en approche progressivement et vient s'appliquer a sa surface, 

 oil il s'aplatit et prend la forme d'une double calotte. En 

 s'appliquant de plus en plus contre le nucleole, il perd son 

 noyau et finit par se reduire a une double membrane qui entoure 

 le nucleole," . . . and finally its substance fuses with that of 

 the nucleolus. 



Hubrecht ('81), egg of Proneomenia : "in all the different 

 stages of development of the ovum the germinal spot is double : 



