No. 2.] COMPARATIVE CYTOLOGICAL STUDIES. 307 



kleinen, gefarbten Kugelchen besteht." In the egg of Anabolia 

 there is one large nucleolus, but in those of Vcspa germanica 

 and V. media apparently no true nucleoli are present. There 

 is a large granular nucleolus in the larger germinal vesicles 

 of Bombiis terristris. Tragus hitorius : there is one large, 

 irregularly shaped nucleolus and two smaller ones ; all these 

 finally disappear, and their place is taken by smaller granules. 

 BancliHs fulvipes : at first no nucleolus is present, later one or 

 three large nucleoli appear, but all of them vanish subsequently. 

 In the egg nucleus of Pimpla sp. only a number of small 

 granules are to be found, and at a later period still smaller 

 granules. Anomalon ciraimjicxtim : in the youngest germinal 

 vesicles no nucleolus is to be found, in older ones there is 

 a single large one ; this has nothing to do in the formation of 

 the " Dotterkerne," and disappears when the nucleus does. 

 There is one spherical germinal spot in Ophion ventricosntn, 

 but not in O. luteum. Ephialtes liturater : in the smaller nuclei 

 a considerable number of " chromatic " bodies occur, while in 

 the older ones there is a single large nucleolus. Avibyteles 

 castigator : one large nucleolus, in older ova also several smaller 

 ones. Epeira diademata : here is one large spherical nucleolus, 

 which later becomes jagged in outline and evinces vacuoles, 

 which may unite to produce a single larger vacuole : " In sel- 

 tenen Fallen kann man einen Zerfall des Nucleolus in mehrere 

 kleinere sehen, was jedoch wohl eine pathologische Erscheinung 

 sein durfte." Glomeris margitiata : one large, spherical or 

 angular nucleolus, and later also a smaller one : " Hochst 

 wahrscheinlich stammt dieser von dem grossen Nucleolus ab " ; 

 the smaller nucleolus disappears subsequently. In the egg of 

 Peripatus edwardsii one nucleolus forms itself gradually, and 

 vacuoles begin to appear in it. In Amaroecium rubiciindiiin a 

 single large nucleolus is present ; while in Clavelina lepadi- 

 fonnis the nucleolus is probably formed out of the central 

 chromatin masses. From these numerous observations Stuhl- 

 mann draws the conclusion : " Aus Allem schien mir hervorzu- 

 gehen, dass das Schwinden des Nucleolus nicht zum Wesen 

 der Eireifung gehort, besonders weil ich ihn bisweilen (so bei 

 Silpha) so lange verfolgen konnte, als noch ein Rest des 

 Keimblaschens im Ei sichtbar war." 



