Phyllophora Brodiæi and Actinococcus subcutaneus. V1 
which produce the spermatia in descending sequence. 
DARBISHIRE calls each filament an antheridium (1895, p. 29). 
These cavities may form a continuous layer or they ap- 
pear singly (fig. 7) and then project more or less over the 
surface. The antheridia were met with in the months of 
March and May to November. They are not restricted to 
the autumn, as supposed by DARBISHIRE. 
For the examination of the procarps the material was in 
several cases treated with FLEMMING’s weaker solution, 
Fig. 7. Phyllophora Brodiei. Two antheridial erypts. A, not fully devel- 
oped. B, ripe, August. 625:1. 
imbedded in paraffine and the microtomed sections stained 
a. m. HEIDENHAIN, but this method has the inconvenience 
that the sections are very liable to loosen from the slide 
owing to the great swelling power of. the intercellular 
substance (Scumirz’s ‘collode’) in water, so that most of 
the sections were lost in many cases. To avoid this, the 
sections were stained with hematoxyline (Mayer’s hemalum 
or Hansen’s hæmatoxyline), or the sexual shoots were 
cut with the freezing microtome and stained with the 
same reagents. Other fixing media used were: formaline- 
sublimate, Nawashin’s treatment', and further formaline 
alone or 70—80 per cent alcohol. And finally, Dr. HENNING 
! See J. CLAUSEN, Chromosome number and the relationship of spe- 
cies in the genus Viola. Annals of Botany, Vol. XLI. Oct. 1927, p. 678. 
Vidensk. Selsk. Biol. Medd, VIII, 4. 2 
