Freeman: observations on chlorocuvtrium. 203 



observation was collected, on the other hand, in summer, May 

 to July. The li<^ht yellow-green color, the absence of repro- 

 ductive bodies and the abundance of small cells point strongly, 

 I think, to the conclusion that this endophyte is but a young 

 stage of C/iIo rocky triuni inclusion Kjellman. A similar dif- 

 ference in the vegetative and resting stages of C. lemnce are 

 recorded in Klebs' observations (^/. jp, f. 2 and p). The 

 form and habit of the endophyte upon Constantinca accord 

 best with Kjellman's description of C. inclusiun ; no mention, 

 however, is made by Kjellman of pyrenoids, which are so 

 conspicuous in the Constantinca material. It is suggested by 

 De-Toni and also by Miss Whitting that Kjellman's species 

 may possibly belong to Chlorocystis, a genus established by 

 Reinhard in 1885. Chlorocystis is described with but one 

 pyrenoid while the endophyte on Constantinca contains many. 

 C. schmitzii, described by Rosenvinge, is but imperfectly 

 known. I have seen in the sections studied a number of cases 

 where the endophyte in Constantinca has assumed approxi- 

 mately the same irregular or obovoidal form shown in the 

 figures of Rosenvinge. The only remaining marine species 

 of Chlorochytriicui, C. dcrmatocolax, lives in the outer mem- 

 brane of Polysiphonia and, according to Mobius, belongs prob- 

 ably to Chlorocystis. • 



The great similarity in vegetative structure to that described 

 by Kjellman for C. inclusttm would indicate that the Constan- 

 tinca endophyte described above is a midsummer stage of C. 

 inclusiim and I would provisionally place it in that species 

 awaiting further information on the life history and develop- 

 ment. 



Bibliography. 



Cohn, F. Ueber parasitische Algen. Remarks before the " Wand- 

 erversammlung der Schlesiscben Gesellschaft fiir vaterliindischer Cul- 

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Kny, L. Ueber eine griine parasitische Alge. Sitz. Gesellch. na- 

 tiirf. Freunde zu Berlin. 1874. 



Wright, P. On a new species of parasitic green alga belonging to 

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Szymanski, F. Ueber einige parasitische Algen. Inaug. dissert- 

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Kirchner, O. Die Algen Schlesiens. Breslau. 1S7S. 



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