274 MINNESOTA BOTANICAL STUDIES. 



maximum daily increase, inasmuch as it occurred later in the 

 evening and during the night at other stages of development 

 of the fruit, is that the translocation of the carbohydrates and 

 proteid substances that go to make up the seed, occurred at 

 this time of day. Further, following a cloudy and rainy day 

 when there had been no decrease nor sunshine, this maximum 

 increase did not occur. 



The grand maximum of daily decrease occurred between 

 8 A. M. and 4 P. m. October 1 5. The increase following this 

 decrease from 4:30-7:30 p. m. was thirty-six grams, or one-half 

 of the total increase from 4:30 p. m., October 15, to 7 a. m., 

 October 16, 



Table XIII. The maximum daily decrease occurred between 

 11 A. M. and 12 M., October 20. 



The plant was sprinkled at 1:28 p. m. when decrease was at 

 the rate of one gram in four minutes. At 1:32, four minutes 

 later, the fruit increased at the rate of one gram in two 

 minutes. This increase, however, continued only three 

 minutes, and at 1 :35 p. m. there was a standstill, immediately 

 followed by a decrease which continued until the regular daily 

 increase began, as soon as the leaves were shaded at 3:30 p. M. 



From 8 a. m., October 15, to 8 A. m., October 22, the total 

 increase exceeded the total decrease by sixteen grams. From 

 8 a. m., October 22, to 8 a. m., October 30, when all increase 

 had ceased, the total decrease was fifty-two grams more than 

 the increase. 



Table XIV. In this table are given data obtained after in- 

 crease in the weight of the fruit had ceased. The object of 

 the prolongation of the experiment was to demonstrate that 

 the daily decrease that had taken place since the grand maxi- 

 mum was chiefly and directly due to the transpiration of the 

 fruit itself, but indirectly to the amount of the transpiration 

 current to the leaves. 



On November 2 the fruit was still in connection with the 

 vine, and showed a daily periodicity in its decrease, which was 

 greatest at the time of highest temperature and least relative 

 humidity. At times when the atmosphere was almost satu- 

 rated, as during rain from 12:30-4:00 A. M., November 2, no 

 decrease took place. With the rise in temperature and fall in 

 the relative humidity, decrease began. 



At 10 A. M., November 2, the vine was cut eighty centi- 

 meters from the fruit and the end of portion attached to it was 

 immersed in a beaker of water. The fruit still remained on 



