XL. CORALLINZZ VER OF PORT RENFREW. 
K. Yenvo, /tigakushi, 
SCIENCE COLLEGE, IMPERIAL UNIVERSITY, TOKYO. 
I had the opportunity of studying seaweeds during the sum- 
mer of 1901, at the Minnesota Seaside Station at Port Ren- 
frew, Vancouver Island, B. C. The vicinity of the laboratory 
is extremely rich in marine life and afforded a good number 
of examples. I paid special attention to calcareous alge, in 
which branch I have been deeply interested. Returning with 
the specimens to the Botanical Institute of the University of 
Tokyo, I carefully examined the Coralline (vere) and pre- 
pared the present paper. Other specimens belonging to the 
subfamily Melobesiz have been sent to Mr. M. Foslie, of Nor- 
way; his paper should also appear in a short time. 
The material was partly dried and partly preserved in alco- 
hol, the accompanying photographs being taken from the latter. 
The sections were made from alcoholic material by microtome, 
after decalcifying in Pereny’s fluid. Amphiroa tuberculosa and 
a few other thick plants were not satisfactorily decalcified by 
the solution and the author found the following mixture specially 
suited for the purpose: 
ydrachionic acid. 5 per cent... 22". < 40 c.cm. 
Paleolials: AWSOMUG 2 om Sus assayed ale ox no oars 20 6.0m 
Guromic, acia,, 0.5 per .cemt.. sc). )./:' ss - BOL eh: 
The sections were stained after my usual method. One 
brings down the sections to pure water, and stains with Boemer’s 
haematoxylin for 20-40 minutes ; treat with acetic acid if neces- 
sary, and then dip in fuchsin (0.3 gr. in 100 c.cm. of 50 per 
cent. alcohol for one hour; go per cent. alcohol, abs. alcohol, 
xylol, balsam. The spores and spore-forming cells stain in red 
and the cell-walls of the vegetative cells in purple. 
711 
