﻿1903] CULTURES OF UREDINEAE 13 



E. nutans Lag. were sown on the same host, and were followed 

 by uredo on July 21, and also on EJiumistrata and -£. margi/iata, 

 with no infection. July 22, uredospores from E, dentata Michx. 

 were sown on the same host, and were followed by uredo on 

 Aug. 14, and also on E. humistrata, E. nutans, and E. mi.r^nata, 

 with no infection. These results, taken in connection with those 

 previously obtained by the writer,'^ leave no opportunity for 

 doubt that the Euphorbia rust is separable into a number of well 

 established races, or possibly into true species. 



2. Uredo rubigo-vera DC. — May 31, uredospores from 

 Triticiim vulgare VilL were sown on Triticum viilgare^ and pro- 

 duced uredo on June 13, and at the same time on Hordeum juba- 



tum^ Poa compressa^ Dactylis glomerata, and Bromus ciliatus^ with 

 no infection. 



3. PucciNiA Peckii (De T.) Kellerm. — The connection of a 

 Puccinia on Car ex trichocarpa Muhl. and Aecidium Peckii De T, 

 on Onagra biemns (L.) Scop, {^Oefiothcra bie?mis L.) has already 

 been reported by Kellerman,^ and it was due to the suggestion 

 from Professor Kellerman, founded upon his field observations, 

 that the writer succeeded in bringing the present season's work 

 to a satisfactory issue.^ Eleven collections of teleutospores 

 were used : two from Ohio, sent by Professor Kellerman ; two 

 from Racine, Wisconsin, sent by Dr. J, J. Davis ; two from 

 Spirit Lake, Iowa, obtained by the writer ; and the remainder 

 from different localities near Lafayette, Indiana. Seven of 

 these collections were definitely known to be on Carex tricho- 

 carpa, and four of them, those from Wisconsin and Iowa, were 

 presumably on that host. 



Spores from these collections were sown on Urtica gracilis, 

 Anemofie canadensis, Gera^dum maculatum, Impatiens aiirea, Sambu- 

 cus canadensis, Ribes gracile, R. Cynosbati and R, floridtim, Xanthitim 

 canadoise, Aster Shortii and A. panictdatus, Solidago canadensis, 

 Erigeron amimis, and Leptilon canadensis, with no infection. Suc- 

 cessful cultures on Onagra biennis were made as follows, the 



BoT. Gaz. 29:271. 1900, and Jour. Mycology 8:51. 1902. 

 5Jour. Mycology 8:20. 1902. 

 For record of previous failures see Jour. Mycology 8: 52. 1902. 



