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BOTANICAL GAZETTE 



[JANUARY 



period between the sowing of the dry spores and the appearance 

 of the spermogonia varying from five to seven da3^s,and between 

 the appearance of the spermogonia and the appearance of the 

 aecidia an equal length of time, the two intervals making ten to 

 thirteen days. 



Cultures of Puccinia from Carex trichocarpa 



ON Onagra biennis. 



Teleutospores 



Spenuogonia 



I ^cldla 



sown 



appeared 



appeared 



April 







26 



1 

 1 



May 







I 



May 7 



May 



2 



May 



8 



May 13 ■ 



May 



2 



May 



8 



May i.S 



May 



2 



May 



9 



May 15 



May 



5 



M ay 



10 



May 16 



May 



7 



May 



12 



May 19 



May 



7 



May 



13 



May 19 



May 



7 



May 



14 



May 19 



May 



7 



May 



14 



May 19 



May 



9 



May 



15 



M ay 2 1 



May 



27 



June 



2 1 



June 7 



May 



28 



June 



2 



June 7 



May 



29 



June 



4 



June 9 



A collection on Carex stipata MuhL also was made near 

 Lafayette, Indiana, spores from which were sown on Ribes Cynos- 

 bati, R. floridiim, R, Uva-crispa, Aster pajiiculahis.A, pre7ianthoides, 



Solidago canade7isis, ^nd S. serotifia, with no infection. It was 

 not until aecidia were found in the field on seedlings of Onagra 

 biennis, growing close to the Carex still bearing dead leaves 

 covered with teleutospores, that the right plant was selected for 

 cultures. Teleutospores were sown on Onagra biennis June 23; 

 spermogonia appeared in great abundance July I, and aecidia 

 Julys. 



4. Puccinia Bolleyana Sacc. and P. Atkinsoniana Diet. 

 The connection of Puccirna Bolleyana, which occurs on Carex 

 trichocarpa MuhL, with Aecidium Sambuci Schw. has already been 

 reported by the writer. 7 This has been confirmed by further 

 successful trials. Material gathered from the type locality of 

 P. Bolleyana, when examined under the microscope, was found 

 to show only a small proportion of the characteristic spores of 



7 Jour. Myc. 8:55. 



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