﻿1903] CULTURES OF UREDINEAE 15 



this species, and a large proportion of much smaller spores, 

 which were surmised to belong to Pucciiiia Peckii, Sowings 

 were made, May 5, on Sambticiis canadensis and Onagra biennis. 

 May 10, abundant spermogonia appeared on Onagra, followed 

 May 16 b}^ aecidia. On Sambucus spermogonia appeared spar- 

 ingly May 13, followed by aecidia May 21. The sedge, there- 

 fore, bore two species of rust in 190 1, intermixed on the same 

 leaves. In addition to this, on May 19, aecidiospores from 

 Sambiicjis cafiadcnsis were sown on Carex trichocarpa^ which gave 

 rise to uredo on May 27, The connection of Pticcijtia Bolleyajia 

 with Aeciditim Sambuci appears to be proven beyond question. 



Two collections of Piiccinia Atkinsofiiana o n Carex liirida 

 Wahl. were sent from Columbus, Ohio, by Professor Kellerman, 

 with the suggestion, which seemed to me at the time very 

 improbable, that the aecidia doubtless occurred on Sambucus 

 canadensis. Another collection was secured from the vicinity of 

 Lafayette, Indiana. Two sowings were made on 0?iagra bie?mis 

 without infection. One sowing from each of the three collec- 

 tions was made on Sa?7tbuc2is ca?iade?isis with abundant success in 

 each case. The dates are respectively April 28, May 7 and 16 ; 

 April 29, May 5 and 16; and May 7, 12, and 21. Professor 



Kellerman has written me that his cultures also gave similar 

 results. 



These discoveries led to a careful microscopic study of the 

 two Carex rusts, and there appears to be no reason to ques- 

 tion that P. Atki?isonia?ia and P. Bolleyana are identical. In the 

 designation of the species it seems necessary on grounds of 

 priority to abandon both these names, however, and it becomes 



Puccinia Sambuci (Schw,), nom. nov. 



5- Puccinia Caricis-Asteris Arth. — A collection of this 

 species on Carex foenea Willd. was sown on Solidago serotina, with 

 no infection. It was also sown. May 2, on Aster paniculatus Lam., 

 and was followed by spermogonia May 8, and aecidia May 19, 

 thus confirming the writer's previous work,^ 



6. Puccinia Caricis-Erigerontis Arth, — A collection of this 



8 



on Carex festticacea Willd 



Jour. Mycology 8 :S4. 1902. 



