﻿1903] 



EXPERIMENTAL STUDIES ON INULASE 



29 



meters of each filtrate being thrown away, and 10*=^ analyzed for 

 levulose by the ordinary gravimetric copper method. The acid 

 solutions without inulase served as controls to show how much 

 of the sugar formed was due to the enzyme and how much to 

 the acid. 



The results are given below : 





No. 



Inulase. 



Reaction. 



Mgms, levulose 



m 



Mgms. levulose 



1 . 









^^^ ^^ ^%^F ^^^^t ^m^F ^» ■ ^-H ^v^H ^V 



m locc. 



due to enzyme. 





I 



Added 



o.oooi N. HjjS04 



65.2 



65.2 





2 



Added 



o.ooi N. H2SO4 



41 



.6 



33.8 





3 



Added 



o.oi N. HgSO^ 



52 



■3 









4 



Added 



0.05 N. H2SO4 



50. 



■5 









5 



Added 



Neutral 



53' 



. I 



53.1 





6 



Added 



Neutral, boiled 



» * 



■ ■ 









7 



* Added 



0.0001 N. KOH 



25 



■3 



25-3 





8 



Adried 



o.ooi N. KOH 



20. 



.0 



20.0 





9 



Added 



o.oi N. KOH 



% % A 



\ % 









10 



Not added 



0.0001 N. HoSO. 



1 t 4 



* 



■ « • p 





II 



Not added 



O.OOI N. HgS04 



7. 



.8 



» ^ ■ * 





12 



Not added 



O.OI N. H^SO^ 



50. 



4 



■ ■ * * 





13 



Not added 



0.05 N. H;,S04 



49. 



1 



■5 



• • * * 



II. The sixteen test tubes used in this experiment were filled 

 in a way similar to that in experiment I. Each tube was corked 

 and kept for twenty-one hours at 35-37 ° C, At the expiration 

 of that time the fluid containing acid were neutralized with 

 barium carbonate in substance. All were then boiled, filtered. 

 and made up to 50^*^, of which 25 '^^ were analysed in each case. 

 Following are the results : 



No. 



Inulase. 



Reaction. 



Mgms. levulose. 



Mgms. levulose 

 due to enzyme. 



I 



Added 



O.OOOI N, H2SO4 



35-2 



35.2 



2 



Added 



0.0002 N. H0SO4 



28, 



■9 



28.9 



3 



Added 



0.001 N. H«S04 



23' 



.2 



23.2 



4 



Added 



O.OI N. H0SO4 



38. 



.8 







5 



Added 



0.05 N. H2SO4 



59 ■ 



,0 







6 



Added 



1 



Neutral 



27, 



.5 



27-5 



7 



Added 



Neutral boiled 



4 « 1 



\ % 







8 



Added 



O.OOOI N. KOH 



26. 



9 



26.9 



9 



Added 



O.OOI N. KOH 



?■ 



,0 



7.0 



lo 



Added 



O.OI N. KOH 











II 



Added 



0.05 N. KOH 











12 



Not added 



O.OOOI N. H0SO4 









13 



Not added 



0.0002 N. H2SO4 









14 



Not added 



O.OOI N. HaS04 









15 



Not added 



pH ^ 



O.OI N. H2SO4 



39. 



3 





16 



Not added 



0.05 N. H3SO4 



1 



59. 



,2 



1 



