﻿30 



BOTANICAL GAZETTE 



[JANUARY 



mgms 



III. Considerable difference is shown between the two tables 

 in regard to the effect of the presence of O.OOOi normal potas- 

 sium hydroxide in the solution. Therefore the following experi- 

 ment was tried. To a solution of inulin Aspergillus powder 

 was added and the fluid shaken. Of this suspension lo^*^ were 

 measured into each of two test tubes; to one lo*^^ of 0.0002 

 normal KOH were added, and to the other lo^"^ of distilled 

 water. Three drops of thymol solution were added to each. 

 After shaking, both were corked and kept in the incubator for 

 fifty-four hours. At the end of that time the solutions were filtered 

 and analyzed for levulose. Solution containing 0.0001 N, KOH 

 gave 32.0"'^'°^; neutral solution gave 36.6 



IV. Experiment IV was tried with the inulase from Pe^ii- 

 cillhim glauctim. The solutions were made up and the tubes 

 filled in a way similar to that in experiments I and II. Each 

 tube, after the addition of five drops of thymol solution, was 

 corked and kept at 38-40° C. for seventy-one hours. At the 

 end of that time the solutions containing acid were neutralized 

 with barium carbonate and all were boiled. After cooling they 

 were filtered through dry filters and each residue washed with 

 10*^*^ of distilled water added in two portions of 5*=*^ each. The 

 filtrate and wash water were united in each case and 25*^^ of the 

 solution analyzed for levulose. The results are given in the 

 table below. 



No. 



Inulase. 



Reaction. 



I 



2 



3 



4 



5 

 6 



7 

 8 



9 

 10 



II 



Added 

 Added 

 Added 

 Added 

 Added 

 Added 

 Added 

 Added 



Not added 

 Not added 

 Not added 



O.OOOI N. H2VSO4 

 O.OOI N. HaS04 

 o.oi N. H2SO4 

 Neutral 



Neutral, boiled 

 o.oooi N. KOH 



N. KOH 

 N. KOH 

 0.0001 N. H2SO4 



N. H2SO4 



M^ins. of levu- 

 lose in 25CC, 



Mgms. of levu 



lose due to 



enzyme. 



O.OOI 

 O.OI 



O.OOI 

 O.OI 



N. HgSO 



-98.5 



31.2 



• # * • 



71-4 



> • ■ • 



64.6 

 19. 1 



* 4 t 



• • * • 



V, In order to ascertain whether the enzyme is destroyed, or 

 only inhibited, by the action of acids and alkalies the following 



