﻿Methods 



Plant Histology 



By CHARLES J. CHAMBERLAIN, A.M., Ph.D., Instructor in BoUny in the University of Chicago 



CONSTANT HELP 



Teachers and Students of Botany 



CONTAINS DIRECTIONS FOR COLLECTING AND PREPARING 

 PLANT MATERIAL FOR MICROSCOPIC INVESTIGATION 



Ir is based upon a course in botanical microtechnique, and is the first complete manual to 

 be published on this subject. It is the result of several years' work with classes in residence 

 at the University of Chicago, and with University Extension classes away from the Uni- 

 versity. It aims, therefore, to meet the requirements, not only of the student who has the assist- 

 ance of an instructor in a fully equipped laboratory, but also the student who must work by himself 

 and with limited apparatus. Free-hand sectioning, the paraffin method, the collodion method, 

 and the glycerine method, are treated in considerable detail. In later chapters specific direc- 

 tions are given for making such preparations as are needed by those who wish to study the 

 plant kingdom from the algae up to the flowering plants. Special attention is paid to the stain- 

 ing of karyokinetic figures, because the student who masters this problem will find little difficulty 

 in differentiating other structures. Formulas are given for the reagents commonly used in the 

 histological laboratory. 



i6o pp., 8vo, illustrated, cloth, {net) $1.50; postpaid $1.59 



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Chicago Press, Chicago, Illinois 



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