136 NATURALISTS’ ASSISTANT. 
solve only a portion of the resulting powder. Let the solution stand 
several days until it becomes saturated and assumes a dark violet color. 
If still too red add more alum. ‘Then filter and dilute with one-fourth 
of seventy-five per cent. alcohol. 
Hematoxylin (No. 2). 
Ground Campeachy wood I Oz. 
Pulverized alum 2 02. 
Mix and triturate ina mortar for twenty minutes; then add two 
ounces of hot aistilled water, and let the whole stand a couple of days. 
Filter and add to each ounce a quarter of an ounce of ordinary alco- 
hol. After standing twenty-four hours more, filter again to remove the 
precipitated alum. ‘This will keep two months in a well stoppered vial. 
Hematoxylin. (No. 3). 
Heematoxylin crystals 35 parts. 
Absolute alcohol 1000 parts. 
Water (distilled) 3000 parts. 
Alum 10 parts. 
Dissolve the hematoxylin in the alcohol, the alum in the water and 
mix. The mixture is purple at first, but gradually turns blue. It can 
however be used at once after filtering. 
Hematoxylin (Kleinenberg’s Method). 
Make a saturated solution of crystallized chloride of calcium in 70 
per cent. alcohol, and add alum until no more will be dissolved. This 
is the first solution; the second is a saturated solution of alum in 70 per 
cent. alcohol. Mix these two solutions in the proportions one of the 
first to eight of the second. Then to the mixture add a few drops of a 
saturated solution of crystallized hematoxylin in absolute alcohol. 
Frey's Fuschine solution. 
Crystallized fuschine I centigramme. 
Absolute alcohol 15 — 20 drops. 
Distilled water I5 cu. centimetres. 
This, though a useful staining medium, possesses the disadvantage that 
it cannot be used where the tissue is to be mounted in balsam. 
