12 The Botanical Gazette. [January, 
\ 
The culture solution used in all cases was a 10per cent. solu- 
tion of grape sugar to which 1 per cent. peptone had been 
added. The above results indicate without exception that 
those germs which were agitated increased from 1.4—2 times as 
_fast as those grown undisturbed. As a check upon the count-- 
ing process, determinations of the dry matter present were 
‘made by chemical analysis at first. 4 
Exp. III, which showed a ratio between A and B of 1.41 
by the counting process gave by chemical analysis the follow- 
ing result. Amount of organic matter formed in A,,, 0.1778 
gm.; in B,,, 0.1293 gm. Ratio between A and B 1.374. 
This proves that the counting process is reasonably exact 
it agrees quite closely with the chemical analysis. | This is” 
only true however where there is general uniformity in size of 
the cells. 1 a 
The amount of alcohol which was produced by this germ 
when subjected to these different conditions, was also detef- 
mined in a number of cases. In every case where this was 
made, a considerable increase in amount of alcohol formed 
was found in the undisturbed culture (B) over the agitated 
culture (A). It would seem then that agitation exerts a favor- 
able influence upon the formation of cells but a retarding effect 
, upon the products of fermentation. Both of these proces 
growth and fermentation, depend directly upon the kineti¢ 
energy of the plant organism® Where katabolic proces es 
are manifested more strongly in fermentative action ther 
seems to be less energy used by the plant in growth. Th 
data of the two following experiments with Monilia candid. 
giving the highest and lowest proportions found by analys 
illustrate this point. i: 
NO. OF REP: MCONCE. PORMED. INCREASED GROWTH OF RATIO. 
: SINGLE CELL, a 
iy 1.6% 335 4 
‘ sia 3.2% 109 Y 
Proportion. 1:2 % 3.0771 * 6.14 | 
; Avi 3.2% 
ae Byr | 3.8% My 
A Proportion. | * Pai narid, 
