1892] Archegonium and Apical Growth in Tsuga and Pinus. 143 
larity, showing that the apex is relatively broad. Transverse 
sections taken from the extreme tip show that it terminates 
in two or three large cells (figs. 6, 7), and it seems to me that 
Wwe can not say with certainty that there is only one initial 
cell. Figs. 6 and 7 are consecutive transverse sections taken 
from the apex. In fig. 7 we have a near approach to what 
would lead one to regard the large cell, x, which has appar- 
ently just cut off a segment, x’’, as the initial cell, both from 
its size and regularity in the arrangement of the cells about 
it. Yet this does not seem sufficient proof to warrant the con- 
clusion. Fig. 5 is the only instance in which I found such 
§reat regularity; in all others the apex terminated in two or 
three large cells, which may be regarded as initial cells, but 
all approached nearly that shown in fig. 3. 
In the apex of the stem of the embryo taken from the seed 
of Pinus sylvestris and Tsuga Canadensis, we find the nearest 
4pproach toa single apical cell (figs. 8, 9 and 10). It is quite 
Probable that in the young state growth takes place from a 
Single apical cell. In instances like that of fig. 8 this seems 
quite certain. In the embryo of Tsuga (fig. 10) this also seems 
‘o be the case, but in fig. 9 we can not be so positive as to the 
initial cell. A transverse section from the tip of the stem in 
aad embryo shows two or three cells of uniform size 
ig 11), 
th : — of these facts it seems to me that we can not say 
at t 
alum cochineal or alum carmine, washed and dehydrated; 
then brough 
t 
microtome. The sections were counter-stained 
; h Bismarck brown. 
‘ana University, Bloomington. 
Meck of Peer hada OF Pate VIII.— F igs. 1, 2 and 3, longitudinal sections showing 
Pinus sy ty gag of Tsuga Canadensis; vc, ventral canal cell. Fig. 4, same o 
of the embryo stem taken from the seed of P. sylvestris. 
soo of Tsuga Canadensis. Fig. 11, transverse section of the extreme 
ear he embryo of P. sylvestris. 
"75 diameters, except fig. 8, 150 diameters. 
