1892. ] Bacterial Investigation of the Sea. 313 
spring and summer of 1891, and at the Marine Bi- 
ological Laboratory at Wood’s Holl, Massachusetts, during 
the past season. 
These widely separated places, so different in many of their 
conditions, gave exceptional advantages for a comparative 
study along these lines. 
Before detailing the results, I will state, in the brief- 
est possible manner, the methods used in the work. 
cork is closed by a glass tube which projects about three- 
then rinsed in sterile water and finally dried on sterile filter 
of the small tube is sealed as the air is expelled. Ln 
‘cuum tube may then be protected from gradual leakage by 
Coati : : 
ating the cork with a mixture of beeswax and rosin. 
