Further Studies on the Apogamy and Hybridization of the Hicracia. 263 



3. H. excellens X aurantiaciim. No. 463. 



The primary hybrid is still alive. With the fiist isolation (in 1905) 

 only one individual of Fo was obtained; it was quite like F,. Isolation 

 was therefore repeated in 1906, and now there came out an F2 of 

 53 individuals which flowered in 1908. Out of these, 52 were homo- 

 geneous and quite like F^, but one individual differed very 

 considerably. This mutant is vegetatively weak and with slight 

 development of stolons, with yellow corollas and with copious pollen, 

 apparently hermaphrodite, while all the other individuals as well 

 as Fj are female i). It resembles much No. 461, but differs with 

 regard to the colour of the corollas, being in the mutant orange- 

 reddish on the underside. Unfortunately it is quite sterile on isolation, 

 which has been tried both in 1908 and 1909. 



F3, sprung from the normal F2, was planted out in 1909, but did 

 not reach flowering. 



We have then here a case where the main part of the second 

 generation of the hybrid behaves as in the two foregoing cases, but 

 where suddenly an individual has come out which is a distinctly and 

 easily recognisable mutant. It is not a retrograde step to any of the 

 parent species, but a new combination of their characters. It is a 

 pity that it is not possible to work further with this mutant, as it 

 seems quite sterile. 



4. K. e xcellensx aiirantiaciim, No. 464. 



From the primary hybrid, which is now dead, was got by isolation 

 an F2 of 27 individuals and by castration 11 individuals, and all 

 38 specimens were quite homogeneous and like Fi. By isolation of 

 one of the 27 individuals of Fg were obtained 107 individuals of F3, 

 and by castration of one of the 11 individuals of F2 were obtained 

 98 individuals; altogether, F3 consisted of 205 individuals, all alike 

 and like Fx. Still, several of them were reddishly tinged and some- 

 what weaker than the others, but this must no doubt be explained 

 in the same way as above. F4 was planted out in 1909, but did not 

 reach flowering. 



I) It is not unknown that a mutant differs from its parent with regard to sexuality; 

 e. g. Oenothera lata, one of de Vries's classical mutants, is purely female while 

 O. Lamarckiana is hermaphrodite. See R. R. Gates (1907a, 1907 b, 1909a) who has 

 worked out its behaviour thoroughly. 



