Ei : 
16 The Botanical Gazette. [January, 
Thus we see that work upon the roots of this order, es- 
pecially as to the meristem, has been very limited. In the 
parts of this paper which follow I have endeavored to point 
out certain types of structure, as I have found them in the 
roots of the native species of Ranunculacee. : | 
University of Chicago. 
‘ag 
jus 
s 
Method for obtaining pure cultures of Pammel’s fungus of | 
exas root rot of cotton. 
GEO. F. ATKINSON. 
It is not a very difficult matter to obtain artificial pure cul- 
tures of spore producing fungi which grow readily in arti 
cial nutrient media. But when we meet with forms of fungi, 
the spore production of which is unknown, quite a serious 
root rot of cotton, described by Pammel in Bulletin no. 7 
of the Texas Agricultural Experiment Station, has not yielded 
to the methods usually resorted to in obtaining pure cultures. 
After trying various methods Pammel failed to obtain a put 
culture. In one case threads of the fungus were swept with a 
camel's hair brush with the hope of obtaining spores. A ‘‘pur 
culture of some fungus” was obtained but its morphologica’ 
characters were unlike those of the fungus found on the root! 
of cotton, . : 
During the summer of 1891, at Auburn, Ala., I m 
several attempts from fresh material received from Texas 
they failed to grow. 
‘Paper presented before the B. : ns 
7 ot : 
and Exp. Sta. New Orleans, Nov. i ceimeseee 
