| 1893.]  nfluence of Anesthetics on Plant Th ranspiration. 59 
2. Petunia violacea. 
first experiment. 
Normal: 6, 5, 5, 6, 6, 5, 5, 5, 6, 5, 6, 5, 5, etc. 
Exposed to ether vapor for 4 seconds: 7 8y:9.:8, 9, 30, 12, 41, ‘ba, EG 8S, 
19, 22, 40, 50, etc., until all motion permanently ceased. 
Second experiment; Jresh sections, 
Normal: 5, 6, Br 2x 5,.5; 5,;5, 6, 5, etc, ; 
Momentary exposure to ether vapor: 5, 5, 6, 7, 7, 7, 8, 8, 9, 9, 8, 9, 8, 9, 8, 
7; ie 7: 6, 6, 5, 5. 
Third experiment; fresh sections, 
Normal: 5, 6, 7, 6, 6-5; Sy 6, 6,: 9, 6, 5, 6, etc. 
Exposed to the ether vapor for one or two seconds: ane ey Oy Pa ay & 
7, 9, 8, 9, II, 12, 14, 15. 16; 16, 
Remained stationary at 16 then returned to normal. 
3. Lycopersicum esculentum. 
Normal: 4, 5, 4, 5, 5, 4, Si Sy: 4B Re, Ole: 
Momentary exposure to ether vapor: 4, 5, 6, 6, 7, 8, 10, 12, 14, 14, 15, 16, 
19,17, I], 16, 17. 
Then returned to normal. 
; From these experiments it will be seen that ether vapor re- 
uced i 
hae effect upon the position, size, or shape of the chlorophyll 
odies. 
III. Experiments on transpiration of entire plants. 
Having noted the effect of ether vapor upon protoplasmic 
activity and believing that transpiration depends on protoplas- 
Not use the entire plant, root and all. In the following ex- 
BB hy have employed a modified and improved Kohl 
slaeeoe apparatus which permits the use of the entire 
Peo, to the fact that this apparatus gives very delicate 
aris ts and hence is easily influenced by slight changes of en- 
en ee the following precautions must be observed in its 
waa must be no air bubbles in any part of tubes J, 4, 
2 
See plate v1 and explanation. 
