1893. ] Bacteria in Vegetable Tissue. 95 
and rollcultures made therefrom. The liquefied gelatine pene- 
trates the section and the bacteria present are able to grow. 
_ Not only were cultures made in this way from the tissue taken 
at the point of inoculation but sections were also taken at 
varying intervals both above and below the point of intro- 
duction. Special care was taken in the use of germ-free 
instruments, so that there was no possibility of transferring 
bacteria from one section to another. 
The results obtained in this way were quite various. 
Nearly all of the species pathogenic for the: animal body, as 
B. anthracis, B. diphtheriae, B. cholerae gallinarum, Micrococ- 
cus tetragenus, M. cereus flavus, Staphylococcus epidermis al- 
bus and St. pyogenes aureus, were killed out in the plant tissues. 
after a lapse of a few days. One notable exception was, how- 
ever, observed in the case of B. pyocyaneus. This germ was 
able to live in healthy plants of different species like Geran- 
lum, Penthorum and Begonia for 50 to 70 days or more and 
even able to spread throughout the plant tissue in an upward 
direction to a distance of 50 to 80™. By far the greater 
humber of the 
ace like B. megaterium and B. lactis aérogenes 
small numb etected in the tissue after 40 days but only in 
Now th ere and only at point of inoculation. 
ese results indicate that bacteria, saprophytes at 
ct 
° grow, to a limited extent at least, if 
the less resistent tissues of the inside : 
4 On thi : : 
| say deta enough data have not yet been gathered to 
4 y, but these observations are presented with ref- 
