tg] The Organism of Leguminous Tubercles. 163 
When the water became low the cork would rest in the neck 
ofthe vessel. The corks were perforated to admit the young 
foots of the vetch seedlings. Mineral fertilizers consisting of 
_ iid phosphate and kainite were added to the water and the 
_ Wessels were then thoroughly sterilized. Vetch seeds were 
geminated on moist sterilized sand, and when the seedlings 
were of sufficient size for the young root to reach the water, 
the plant was placed in the perforation of the cork. Three 
plants were transferred to each vessel. In a few days when 
_ ‘itwas assured that the plants were all growing nos. 1 and 4 
Were inoculated by transplanting with a looped platinum 
oh : quantity of the organisms from the pure culture to 
| Sela of the seedlings in the perforated cork. This ex- 
i hoa also successful and though the tubercles were 
‘The eit So distinct as to be readily photographed. 
b ediad ie DS aaa was then photographed and is repre- 
E Msbo- ota Two tubercles can be seen in each of 
anc’ 4. The checks, nos. 2, 3 and 5 possessed no 
E tetlisent st oo used in this second inoculation ex- 
: € of the fifth transplanting in the culture tubes. 
ee OSanism descri 
Media the Specific m 
Mode of infection. 
€ material which formed the basis for 
nism in its natural relation to the tuber- 
: Ae Stated above, th 
Study of the orga 
Was obtained fro 
only a low convex lateral 
e side as shown in figure 4 
possessed numerous normal 
bent in the 8 undergone further change in shape by 
o- Set OF @ shepherd’s crook or a walking 
fganism y 
Sually enters at the end of the root 
