228 The Botanical Gazette. 
the plants did not fruit could themselves develop into perfect 
ants. According to Nobbe,?° Lachman, ?® Mattirolo eBus 
calioni?? they were places for the storage of reserve food — 
materials. . 
These notions were probably empirical and derived mor — 
from superficial examination than from any serious attempt — 
at an anatomical study of their structure and contents. 
Middle period of tnvestigations. 
chymatous tissue which is separated into an inner central a 
mass and an outer layer, the two portions being separated by 
a layer of fibro-vascular tissue which arises from corer — 
ponding tissue in the central cylinder of the root. The 
inner parenchyma he described as possessing turbid com 
tents while the outer was clear. The growing point ® 
meristem of the tubercle lies in the distal portion of the ! 
‘teria-like bodies or vibrios which filled the plasmic substance 
These he considered to be the cause of the tubercles. ue 
The next important contribution was by Eriksson” 
distinguished what he supposed were two organisms eS: 
tubercles. One he verified as the vibrio-like bodies disco 
central parenchymatous tissue. In the distal po 
central parenchymatous tissue he discovered 
be fungus strands lying in a radial position, 
finer threads as they approached the center. 
1856, p- 37: 
offen. Landwitt 
?5Vegetationsversuche in Boden mit lokalisirten Nahrstowes. 
schaftliche Versuchstationen, 1868, p. 98. hei) Zeitscht- 
26Ueber Knollchen der Leguminosen. Landw. Mittheil. Cited 
Lehranstalt und Versuchstationen, Poppelsdorf, 
Tschirch, j. c. 
ih. Sporn ane bacteri nei tubercoli radicati delle Leguminose * r 
1, 21, 464, cited by Pichi, 1. c. a 
*®Ueber die bei der Schwarzerle und der gewoebnlichen me Lupine : 
oto Wurzelanschwellungen, Mem. d. I’ A cad. Imp. Sciences pe 
: Bot, Zeituas 
*®Studier Sfver Leguminosernasrotknélar, Lund, 1874; 
P. 381. Cited by Vuillemin, 1. c. and others 
