246 The Botanical Gazette. 
i 
Since my results agree closely with those of Strasburger : 
upon Senecio vulgaris a brief résumé of the latter may be for 
convenience given here. 4 : 
As will be indicated below, I am here concerned only with 
the nearly mature and mature embryo-sac, and such factsonlyas _ 
pertain to it will be mentioned. In the nearly mature em- 
bryo-sac the.two synergidz occupy the entire forward pointed 
end of the embryo-sac. Under these lies the egg, occupy- 
ing usually the entire diameter of the embryo-sac. The 
antipodal cells completely fill the hinder end of the embryo-sat. 
They lie in a longitudinal row, or two above and one below. 
The lower antipodal cell gives rise to a large hinder vacuole 
asacelllumen. Its nucleus lies in the protoplasm lining the 
wall, at about one-half the height of the cell lumen. Thetwo — 
nuclei given off to the inner part of the embryo-sac approach 
each other quickly to unite just as the embryo-sac approaches 
maturity. When this state of maturity is reached the em 
bryo-sac swells considerably at its middle part. Thesy nergide 
have now rounded themselves off somewhat at the forward 
end, and appear here strongly refractive. Their nuclei lie 
the hinder third. By the swelling of the embryo-sac, the egg 
has become free at its hinder part. The lowest antipodal 
cell usually divides once before the mature state Ist " 
so that there are now four antipodal cells. Frequently, ie 
ever, only the nucleus divides without being followed by 
cell division. 
The embryo-sac. 
In preparing the material for this work heads yer 
were gathered in the stage of developmen ight) it 
and treated for about twelve hours (usually pee ” After 
a one per cent. aqueous solution of chromic act ee a 
thorough washing, they were gradually brought m toto with — 
five per cent. alcohol. The material was stained 98 and 
alum cochineal, imbedded (through xylol) in vere cout 4 
sectioned on a Minot microtome. The sections s 
_ter-stained on the slide and mounted in baer of fore 
*Angiospermen und Gymnospermen, 1879, pp. 9 1% at i : 
Zelltheilung, dritte Auflage, 1880. pp. 42, 43- 
