idl, 
14g.) Embryo-sac and Embryo of Senecio. 247 
sacs. We are concerned here with the mature embryo-sacs, 
and with that stage in the development just before the nuclei 
given off to the interior, unite to form the endosperm nucleus. 
The earlier stages in the development observed agree with 
that which obtains in Senecio vulgaris according to Stras- 
burger and will not be repeated here. 
The two synergidae and egg-cell occupy the somewhat an- 
terior pointed end of the embryo-sac (figs. 2; °3; 8; 9902 ine 
synergidee are somewhat pear-shaped, being narrower at the 
anterior end, where they are also rounded. In all cases ob- 
served the anterior ends of the synergidz never appeared re- 
fractive, but filled- with granular protoplasm which stained 
only slightly with alum cochineal, but stained readily with 
Bismarck brown. The posterior part of each of the synergide 
® Sccupied by a large vacuole which reaches fully half the 
length of the cell and probably extends farther forward than 
# seems. The vacuole does not take up thé entire posterior 
shag cell, but a delicate layer of protoplasm forms an in- 
toohane ct the cell. The nuclei lie imbedded in this pro- 
eg lining about midway between the anterior and post- 
third ends. In no case were they observed in the posterior 
- Strasburger states for Senecio vulgaris. oe 
fied vo is similar in form to the synergidz, though in- 
a ittle lower, the posterior part occupying, in the many 
Sologlases i the entire diameter of the embryo-sac. : The 
imbedded ‘te accumulated in the posterior end in which is 
i . ea while the vacuole is in the oe 
i ous in the egg-apparatus there is a sort of equili- 
Richens pentained in the distribution of the protoplasm. The 
coking 7 tee nucleolus which stains so deeply with alum 
Variably aS to appear a homogeneous red, except there is in- 
;. Present from one to several highly refractive bodies 
Products of the 
10), ably 
< In near ly all D 
When t 
so em eryO-sac is mature it swells considerably at its 
abe €gg-apparatus is free behind. 
