Dea a eee 
a : Cc Nnged 
1893.] Embryo-sac and Embryo of Senecio. 249 
beautiful net-work of protoplasm seems to radiate from the 
region of the egg nucleus, though between it and the ob- 
server, almost obscuring the endosperm nucleus lying be- 
neath, 
Fertilization. 
Senecio aureus is by no means a very favorable object for 
the study of the process of fertilization. As is well known, 
the dicotyledons as a rule, present here many difficulties. 
Either the ovules are Opaque, necessitating sectioning, or the 
nuclei in the pollen tube as well as the tube itself are extremely 
small. In Senecio aureus and the Composite in general both 
mg difficulties confront the observer. However, by the 
aid Of a 2™™ 
NE Just as it had reached the anterior ends of the syner- 
sad hen 8, 9, 10). Its membrane is extremely delicate 
The ai € collapses soon after the contents have left it. 
synergid ents now spread out upon the anterior ends of the 
a re Ree them appear more densely granular (figs. 
nocare .. Senerative nucleus as it leaves the pollen tube, 
appears ‘ : 
substance Pigs small and slightly stained mass of nuclear 
Paratyus ei nourished by the contents of the egg ap- 
ence of a polle © Preparation represented in fig. 9, the pres- 
the sy 
ra ga densely granular, but no definite nucleus could 
= 
: j . 
shilaed «4, of the micropyle just in front of the synergide 
Sesting at megeneous brown (not shown in the figures), sug- 
Which in ane the presence of a mucilaginous substance, 
10 
the ace tobability is secreted by the synergide. In fig. 
Cleus, ative nucleus is seen lying close to the egg-nu- 
Even II, two, though smaller, nuclei are seen. 
g. 
at thi : . 
little ae ; ‘age in the Process, the synergidz seemed very 
’ “xcept their membranes were extremely del- 
