1893. The Organism of Leguminous Tubercles. 259. 
cultures, especially from tubercles of Lupinus, numerous 
larger bacteroid forms developed, ‘‘Wir haben jedoch in un- 
seren mehrfachen Uebertragungen Gebilde oft in grosser Anzahl 
gefunden, welche unzweifelhaft als echte Bakteroiden ange- 
sprochen werden mussten.” 
The tests imposed by Eriksson,!°7 Ward,1°8 Vuillemin, }°9 
Pichi,’*° A. Koch,111 and Laurent!12 for the determination 
of the presence of a membrane in connection with the thread- 
like structures, would seem to impeach Prazmowski’s''* and 
Frank's!" explanations of its nature. H. Mdller!+® also finds 
amembrane on the strands but interprets it as being a cellulose 
membrane deposited by the protoplasm of the leguminous 
plant around the bacterian zoogloea, and cites in support of this 
view cellulose membranes said by R. Wolff?!® to be deposited 
— the threads of Ustilaginee by their hosts. Such a view 
of th ie seem to be any greater proof of the bacterian nature 
U: le organism of the tubercles than of a like nature for the 
stilaginee. 
itt also to harmonize Prazmowski’s description 
majorit a. in artificial cultures with that obtained by the 
the he ose who have succeeded in growing it outside of 
cultures a Tt would, perhaps, be unjust to infer that his 
mitting oaks contaminated; or shall we suspect him of com- 
hess "sat error which he imputed to Beyerinck, 772., 
Condition of . in the appearance of the forked or lobed 
Might be : Organism? Prazmowski says this appearance 
since fre ‘degra by one rod lying partly over another, 
anc an y he was for a time deceived by such appeat- 
bacteroids are We not then, on the same ground say that the 
t are not forked? 
was im 
the pre 
TMs were exceedingly rare. It would seem 
“rent’s work was carefully guarded for Metchnikoff 
fer ‘ 
. Soc. cLxxvit; Proceed. Roy. Soc. XLVI. 
Ati g Sci. Tons hora etetrang. 1888 é 
: i 88. 
‘ nl Cl. nat: i : 
Aan, d. ey der Faden in den Wurzelknéllchen. Bot. Zeit. 1890. 
1). 
t 1890). 
oe der Leguminosen. Berlin, 1890. 
d. Getreides, Pag . X (1892), 242-249. 
