ctl) 8 
bi oa a 
1893] 
The Organism of Leguminous Tubercles. 264 
probably be more nearly correct to say that the death of the 
organism, in its passage to the sterile condition of the perfect 
bacteroids is first indicated by a firmer condition of the or- 
ganism, probably brought about by the increasing presence 
of proteid matter which in many cases finally becomes cen- 
tered in different parts of the bacteroids and forms bodies 
which possess a very high power of refracting light. Lund- 
strom'*? described these in the tubercles of 7; rifolium repens. 
In some cases these bodies occupy nearly the entire inner 
portion of the bacteroids and frequently the accumulation 
bodie 
se ee | violet. The present writer has observed 
Oe staizs ®not take the stains gentian violet and fuchsin. 
With tte bacteroids from tubercles of Medicago denticulata 
in this res in they present an interrupted stain, simulating 
likely th pect the rods of Bacillus tuberculosus. It is quite 
“eos difficulty experienced in staining these objects 
int 
as = cles has led some to describe the stained portions 
e ay 4 rose red color, which he claims shows them 
the starch oh bodies Recently Frank!!° places them with 
Miller 129%. UP Calling them amylodextrin bodies while H. 
tetin. | — Says they represent some form of choles- 
‘OVered a gi = oe Paper Frank states that he has dis- 
that the lane nism in the tubercles on the roots of peas, 
'8€ profusely forked ones bear principally these 
Bot Con 
dea Di hismus der Wurzelknéllchen der Erbse. Berichte d. 
ert bot. 
wtBemericane X (1892). 170-178, 
“MSBlichen deere co Frank's Mittheilung tiber den Dimorphismus der Wur- 
Vol - Ber. d. deut. bot. Ges. x (1892). 242, 249. 
XVIII ~ No, 4. 
og MII (1888), 
