1893.] The Organism of Leguminous Tubercles. 263 
oped tubercles. Phaseolus vulgaris inoculated with cultures 
from tubercles of Phaseolus and peas developed tubercles, 
but if inoculated with cultures from tubercles of Lupinus or Ro- 
binia, none were developed. In one case Pisum sativum in- 
oculated with lupine tubercle organisms developed tubercles, 
while in other cases it did not. In the case where the tuber- 
cles were developed, the hyphz and bacteroid characteristic 
of those of the peas under normal conditions were developed. 
Ifthis development of tubercles on peas from lupine organ- 
sms were not an accidental contamination it would indicate 
that one and the same species occurred in the tubercles of 
peas and lupine. Other cross inoculations made by them oc- 
casionally took effect but there was shown a disposition to 
tardy and weak development as if the organism had been in 
some unsuitable condition. 
At the same time that the present writer carried on the 
second experiment in the inoculations of Vicia sativa with 
“kage cultures of the vetch tubercle organism, inoculations 
Te also made of young plants of Dolichos sinensis with or- 
ganisms from the same culture but no tubercles were devel- 
; while the inoculated plants of Vicia sativa developed 
ubercles, 
atte the almost universal infection of legumin- 
: when grown in a state of nature it is difficult to 
le nt upon the microsymbiont while within the tu- 
* @ certain type of racial form and attenuation upon 
*onditions in ip until it shall have passed through normal 
ie Maintaines 2 the organism is at first so virulent. W me 
Cially so ; = eta! Wil 
Oduced y some that these racial peculiaritie 
