266 The Botanical Gazette. 
Little value is attached by the writer to these observations — 
since it was impossible at that time to repeat them, for ina ’ 
few days his labors were to be removed from Alabama to 
New York, and during the move the infusion of Vicia sative : 
with which it was designed to make media to prosecute the 
study farther became contaminated. During the busy period 
of organizing work in a new field the organism died before | 
fresh culture media could be made. ‘a 
Laurent did not observe a motile stage. Beyerinck ob- 
served motile forms which agree in size with the smallest 
forms (0.2) obtained by myself. If Frank’s micrococci and 
Prazmowski’s swarmers could be regarded as the same forms 
represented by these small individuals the possibility of there 
being zoospores would be strengthened. Should the present 
of zoospores be confirmed it would indicate a relationship © 
the lower Phycomycetes. It will be remembered that Vuille- 
min (l. c.) placed the organism in the Chytridiacea, but the 
zoospores of his Cladochytrium tuberculorum were 7# 1 
eter, a size much greater than any except possibly 
the very largest of the organisms obtained by other investis 
tors, and since his studies were made in late autu me 
tubercles there may have been some chance of mona | 
curring in the tissue of the tubercles. 
ism is very much like Cladochytrium tenue,ye “a 
sential characters it departs too widely from ss oe 
that even if it should eventually be clearly shown to 
the Chytridiacee, it would still be referable to Phytomys rs 
Cornell University. 
EXPLANATION OF PLATES XII-XV. 
PLate XII.—Tubercles of Vicia sativa, from photographs 
Piate XIII.—Fig. 4. Young tubercle, rmagmiies 
Fi i s 
dodermis; fe, pericambium; £4, phloem; +, xylem. o¥i 
LATE x1 Fi .9. Infecting thread drawn to lat 
of bacteroid tissue containing branching threads nee i cA 
bercle cells.—Figs. 11 and 12. Organisms in pu 
tc 1 i : 
%4™™, and the objects are magnified thirty times mo tiva. ‘s 
LATE XV.—Inoculation. Water culture 0 Pee Piytomyxe from 
oculated with organisms from pure culture 0 
tubercles. From photograph. 
