416 The Botanical Gazette. [November, 
tic at least. Aside from this species, another mud form was 
secured on the Grampus that bore a very close resemblance 
to B. granulosus, a prevalent Neapolitan species. Careful 
comparison on same culture media of the germs from the two 
sources showed only slight differences, but these were scarcely 
more than would be expected of specimens coming from 
widely separated localities. 
Concerning the distribution of those species that are com- 
mon to this locality but unknown in the Mediterranean bot- 
tom, some observations are to be noted. First there is 4 
marked difference as to the habitat of different species. Some 
are found solely in the mud layers, others are common to both 
water and mud. : 
Bacillus limicola, a common chromogenic species inhabits 
exclusively the ground layers of the sea bottom. In the vicin- 
ity of Woods Holl, it is a common form and was found in 
shallow places adjacent to the coast as well as in the deeper 
mud layers at a distance of ten to fifteen miles from land. 
Three other forms, B. pelagicus, B. maritimus and B. ie 
osus which comprise, with the above mentioned species, t ' 
major portion of the bacterial flora of this locality, hea 
in both the water and the underlying ground layers. 4B 
were distributed throughout the water at all depths a 4 
pelagicus and B. maritimus were abundant in the samp pee 
mud taken on the Grampus at a distance of 100 miles 
land and in 450 feet of water. 
Karlinski}® has put forth a theory based upon the ex hor- 
tion of one of the fresh water lakes in Bosnia that there is pel 
izontal zonary distribution of bacterial forms in water S rs, 4 
He found,even within a vertical range of seventeen pene 
marked separation of the bacteria according to “f* the 
Forms common at the surface disappeared entirely } 
deeper layers and were replaced by species Pp — btained at 
merged strata. These results contravene those a sie bath- 
both this locality and at Naples. Here the study limited on 
ymetrical range of the different species has been % ree” 
account of the shallowness of the sea, but the op pete two 
forded by the dredgings from the Grampus paesees a 
forms, B. pelagicus and B. maritimus, were ghee banks 
ance at the depth of 450 feet as well as in the mu 
the shore. ¢ this question 
Much more favorable conditions for studyin 
23To¢. cit. 
amina- 
. 
é 
