py Bacterial Flora of the Atlantic. 441 
ten seen individual cells, having one end pointed, or lance- 
“type, and have a more refringent aspect that closely re- 
oils the appearance of arthrospores of some species, but the 
tllunion of these forms would hardly be conceivable with 
res, 
. Then, too, the fact that old non-sporogenous cultures, hav- 
MG these refringent cells, were killed at the comparatively low 
‘“mperature of 55°C. is against the view that they are genu- 
‘pore stages of this bacillus. 
ether the wide variation in form and size is due to the 
orphic nature of the species or to involution changes 1s 
‘tion. These appearances are to be noted in cultures 
old and in hanging drop preparations the unstained 
‘la have the ordinary optical properties of active proto- 
» but when subjected to staining agents like methylene 
— °F carbol-fuchsin, they stain so irregularly as to lead one 
ko, Cc that a degeneration of the protoplasm has epee 
Cover-glass preparations stained by Grams niet 
decolorized. 
‘the germ was first isolated at Woods Holl, spores 
€n observed in agar cultures kept at ordinary summer 
‘mg, Mt in later cultivations on both salt and fresh 
Temarkable f this p 
» yet the observance o : 
other bacterial forms like anthrax when cultivated on 
% 
