444 - The Botanical Gazette. [December, 
and in cultures prepared according to Buchner's pyrogallic — 
method, it does not develop. 
The liquefaction of the gelatin in ordinary tube cultures — 
progresses slowly until the side of the tube is reached an 
then continues steadily to the bottom in horizontal layers. 
In old cultures, the film on the surface often has a reddish 
hue. The appearance along submerged portions of the needle 
track indicates only a scant growth. 2 ; 
Streak cultures on agar show a thin hazy film becoming 
more opaque in lower part of tube where condensation water 
collects. On agar plate cultures, considerable variation is to 
be noted. Sometimes the colonies are small and opaque, 
then again they are often spread out in a thin film as in tubes. 
The colonies on agar are more or less viscid, and adhere . 
easily to the needle when touched. : 
On potato, this bacillus is a chromogene and grows more: 
luxuriantly on this than any other medium. In thirty-six — 
hours, at room temperature, the culture growth 1s apparent — 
where it appears as a glistening yellowish drop around parietal 
lation puncture. rake 
This rapidly spreads until the whole surface of potato : | 
often covered with a deep orange-yellow growth that change 
later in old cultures to a brick red. db 
Milk is modified profoundly, the casein being coagulate’ 
the production of acids. This coagulated material 1s $ 
peptonized, the serum remaining more or less cloudy. ee : 
Bacillus litorosus, sp. nov.—This form was found ei 
water and mud layers in not inconsiderable numbers, 4 = 
not nearly so common as B. pelagicus. i 
Morphologically, it belongs to the larger type of pest, 
being a slender, typical bacillus form (1 * 41 uae 4 
usually found as an isolated individual or closely in aa: 
short chains of two or four segments. The provera sad 
the cells is usually homogeneous. This form 1s pe verpele 
is characterized by a slow, stately movement o! 4 . 
tine nature. : ristic 
_ In ordinary gelatin media, it forms quite 4 charact® 
culture. Two or three days after inoculation, 
gelatin is to be noted at the surface and along nee lace more 
well. The peptonizing of this substance takes ie formed 
rapidly at surface, so a broad funnel shaped sac is usually 
as a result of two or three days’ growth. There 
